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Fibroblasts/Epithelial cells Antibody (D7-FIB) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, IHC-P (-)
Clone
D7-FIB
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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Fibroblasts/Epithelial cells Antibody (D7-FIB) [FITC] Summary

Immunogen
Human foreskin fibroblasts
Localization
Cell Membrane
Specificity
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

0

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for Fibroblasts/Epithelial cells Antibody (D7-FIB) [FITC]

  • fibro-blasts

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Fibroblasts/Epithelial cells Antibody (NB600-777F) (0)

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Secondary Antibodies

 

Isotype Controls

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