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Fibroblast Antibody (ER-TR7) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
ER-TR7
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594

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Fibroblast Antibody (ER-TR7) [Alexa Fluor® 594] Summary

Immunogen
Isolated C3H thymic stromal cells
Specificity
NB100-64932 recognizes ER-TR7, an antigen that is located in the cytoplasm of reticular fibroblasts and is a component of the extracellular matrix of lymphoid and non-lymphoid organs. The antigen recognized by clone ER-TR7 has not been identified but studies suggest that it is likely to be distinct from laminin, fibronectin, collagen types I-IV, heparin sulphate proteoglycan, entactin and nidogen. Clone ER-TR7 has been used to stain the microanatomy of various organs and also stains subendothelial deposits in atherosclerotic plaques.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Fibroblast Antibody (ER-TR7) [Alexa Fluor® 594]

  • reticular fibroblast

Background

A fibroblast is a cell that synthesizes collagen and the extracellular matrix. Inactive fibroblasts are known as fibrocytes. Fibroblasts are important in healing wounds, and their mitosis is induced by tissue damage. Fibroblasts can be identified by their high levels of rough endoplasmic reticulum. They are also the most common connective tissue cells in animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Fibroblast Antibody (NB100-64932AF594) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Fibroblast Products

Array NB100-64932AF594

Research Areas for Fibroblast Antibody (NB100-64932AF594)

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Blogs on Fibroblast.

IRE1 alpha Regulates Dendritic Cell Function in Response to Viral Infection
By Natalia Gurule, PhD What is IRE1 alpha?Inositol- requiring enzyme type 1 alpha (IRE1a) is a serine/threonine kinase that is one of the three transducers within the unfolded protein response (UPR) signalin...  Read full blog post.

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