Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein Summary
| Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-168 of E.coli Double-Stranded Uracil-DNA Glycosylase Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 20% glycerol |
| Preservative |
No Preservative |
| Concentration |
0.5 mg/ml |
| Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant E. coli Double-Stranded Uracil-DNA Glycosylase His Protein
Background
G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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