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Human Colon ascending Whole Tissue Lysate (Adult Whole Normal)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
5.0 mg/ml

Order Details

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Human Colon ascending Whole Tissue Lysate (Adult Whole Normal) Summary

Description
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
Specificity
Total Protein - Human Adult Normal Tissue: Colon Ascending

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Digestive/Waste
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional Colon ascending Products

Array NB820-59206

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