CD9 Immunobeads for Exosome Isolation Summary
| Description |
CD9 Immunocapture Beads are 0.4 um latex beads covalently coupled with antibodies against extracellular vesicle (EV) surface antigen CD9. Samples containing EVs are incubated with Immunocapture Beads for rapid and specific isolation of EVs expressing CD9.
Using the supplied Elution Buffer, EVs can be eluted from Immunocapture beads and used in downstream analyses such as protein profiling and nucleic acid extraction.
Immunobeads can be re-used after incubation with Bead Regeneration Buffer. |
| Sample Volume |
0.5-1 mL |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- CD9 Immunocapture BeadsExosome Elution BufferBead Regeneration Buffer
|
Alternate Names for CD9 Immunobeads for Exosome Isolation
Background
Immunocapture for isolation of exosomes relies on the antigen-antibody interaction. Antibodies directed at target of interest (eg CD9, CD63) are coated either in a 96-well plate or conjugated to a bead. The sample is applied directly into coated well, or into sample with coated beads, and EVs are allowed to bind. In a final step, EVs can be eluted from the antibody and used in downstream functional and phenotypic analysis. This method of isolation is highly specific, resulting in extremely pure EV populations.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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![CD9 Immunobeads for Exosome Isolation [NBP3-11768] - Anti-Alix WB analysis on exosomes immunocaptured with beads for overall (NBP3-11768) and for tumor-derived exosomes (NBP3-14798). WB shows the capture of exosomes only for cancer patients when beads for tumor-derived exosomes are used.](http://images.novusbio.com/fullsize/nbp3-11768_cd9-immunobeads-for-exosome-isolation-17122024156304.png "CD9 Immunobeads for Exosome Isolation [NBP3-11768] - Anti-Alix WB analysis on exosomes immunocaptured with beads for overall (NBP3-11768) and for tumor-derived exosomes (NBP3-14798). WB shows the capture of exosomes only for cancer patients when beads for tumor-derived exosomes are used.")
![CD9 Immunobeads for Exosome Isolation [NBP3-11768] - Alix expression by western blotting of human plasma exosomes captured on lmmunobeads from 0.5 ml of plasma in comparison with exosomes purified via ultracentrifuge. 1, 3 and 5: lmmunocapture with immunobeads from 1 ml, 0.5 ml and 100 µ1 of human plasma respectively. 2, 4, 6: Ultra centrifuged exosomes from plasma after immunobeads capture. 7 lmmunocapture with immunobeads isotype coupled. 8, 9: Ultracentifuged exosome from 0.5 ml and 1 ml of human plasma.](http://images.novusbio.com/fullsize/nbp3-11768_cd9-immunobeads-for-exosome-isolation-17122024156302.png "CD9 Immunobeads for Exosome Isolation [NBP3-11768] - Alix expression by western blotting of human plasma exosomes captured on lmmunobeads from 0.5 ml of plasma in comparison with exosomes purified via ultracentrifuge. 1, 3 and 5: lmmunocapture with immunobeads from 1 ml, 0.5 ml and 100 µ1 of human plasma respectively. 2, 4, 6: Ultra centrifuged exosomes from plasma after immunobeads capture. 7 lmmunocapture with immunobeads isotype coupled. 8, 9: Ultracentifuged exosome from 0.5 ml and 1 ml of human plasma.")
