![Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Primary antibody dilution- 1:1000 for 2 Hours at RT, in blocking buffer, 5% Milk in TBS-0.05% Tween : Blocking Overnight at +4 degree C. Secondary antibody : 1:2000 in blocking buffer, 1 hour at RT. Image from verified customer review.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Western-Blot-NB120-2864-img0014.jpg "Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Primary antibody dilution- 1:1000 for 2 Hours at RT, in blocking buffer, 5% Milk in TBS-0.05% Tween : Blocking Overnight at +4 degree C. Secondary antibody : 1:2000 in blocking buffer, 1 hour at RT. Image from verified customer review.")
| Reactivity | Hu, Mu, Rt, Po, Gp, RbSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | 20A |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified rabbit dihydropyridine receptor. |
| Specificity | Detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from skeletal and cardiac muscle. Detects a band at 220 kDa under non-reducing and a 143 kDa band under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with NB120-2864 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CACNA2D1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Use in immunoprecipitation reported in scientific literature (PMID: 8070639). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.05% Sodium Azide |
| Purity | Unpurified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Anmol Kumar |
WB | Human | 06/06/2018 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Immunocytochemistry-Immunofluorescence-NB120-2864-img0005.jpg "Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.")
![Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized mouse skeletal muscle tissue.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Immunohistochemistry-Paraffin-NB120-2864-img0002.jpg "Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized mouse skeletal muscle tissue.")
![Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of C6 cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Flow-Cytometry-NB120-2864-img0012.jpg "Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of C6 cells compared to an isotype control (blue).")
![Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Western-Blot-NB120-2864-img0013.jpg "Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates.")
![Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Immunocytochemistry-Immunofluorescence-NB120-2864-img0003.jpg "Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.")
![Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Immunocytochemistry-Immunofluorescence-NB120-2864-img0004.jpg "Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.")
![Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized human skeletal muscle tissue.](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Immunohistochemistry-Paraffin-NB120-2864-img0001.jpg "Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized human skeletal muscle tissue.")
![Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of SH-SY5Y cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Flow-Cytometry-NB120-2864-img0010.jpg "Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of SH-SY5Y cells compared to an isotype control (blue).")
![Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of Neuro-2a cells compared to an isotype control (blue).](http://images.novusbio.com/fullsize/CACNA2D1-Antibody-20A-Flow-Cytometry-NB120-2864-img0011.jpg "Flow Cytometry: CACNA2D1 Antibody (20A) [NB120-2864] - Analysis of Neuro-2a cells compared to an isotype control (blue).")
