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Bromodeoxyuridine/BrdU Antibody (85-2C8) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Flow, ICC/IF, IHC, ICC/IF
Clone
85-2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

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Bromodeoxyuridine/BrdU Antibody (85-2C8) [Janelia Fluor® 635] Summary

Immunogen
Bromodeoxyuridine/BrdU conjugated to hemocyanine (isolated from Helix pomatia)
Localization
Nuclear
Marker
Proliferation Marker
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Bromodeoxyuridine/BrdU Antibody (85-2C8) [Janelia Fluor® 635]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-92988JF635). (Showing 1 - 1 of 1 FAQ).

  1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
    • We recommend this product: NB500-235​

Secondary Antibodies

 

Isotype Controls

Additional Bromodeoxyuridine/BrdU Products

Blogs on Bromodeoxyuridine/BrdU.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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