![Flow Cytometry: Recombinant Fungi Alternaria alternata 1.0101 Strep (N-Term) Protein [NBP3-06716] - Flow cytometry dot-plot staining pattern of Alternaria alternata 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.](http://images.novusbio.com/fullsize/Recombinant-Fungi-Alternaria-alternata-1-0101-Strep-N-Term-Protein-Flow-Cytometry-NBP3-06716-img0001.jpg "Flow Cytometry: Recombinant Fungi Alternaria alternata 1.0101 Strep (N-Term) Protein [NBP3-06716] - Flow cytometry dot-plot staining pattern of Alternaria alternata 1.0101 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (NB100-77913) FITC and anti-human CD203c PE antibodies.")
| Reactivity | FuSpecies Glossary |
| Applications | ELISA, Flow, PAGE |
| Description | A recombinant protein with a N-Terminal Strep-tag corresponding to 152 amino acids of Alternaria alternata 1.0101 Source: E.coli |
| Preparation Method |
The protein was purified by ionex and affinity chromatography, using Strep-tag. Endotoxin was removed using a specific endotrap carrier. Product was lyophilized after purification. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Purity | >95%, by HPLC |
| Dilutions |
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| Theoretical MW | 16.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from a 0.22 um filtered solution in Tris buffer (100 mM Tris, 150 mM NaCl, pH 8) |
| Preservative | No Preservative |
| Purity | >95%, by HPLC |
| Reconstitution Instructions | Reconstitute with sterile deionized water with or without azide. |
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![SDS-Page: Recombinant Fungi Alternaria alternata 1.0101 Strep (N-Term) Protein [NBP3-06716] - Recombinant allergen Alternaria alternata 1.0101 purity verification. 5 ug of Alternaria alternata 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.](http://images.novusbio.com/fullsize/Recombinant-Fungi-Alternaria-alternata-1-0101-Strep-N-Term-Protein-SDS-Page-NBP3-06716-img0002.jpg "SDS-Page: Recombinant Fungi Alternaria alternata 1.0101 Strep (N-Term) Protein [NBP3-06716] - Recombinant allergen Alternaria alternata 1.0101 purity verification. 5 ug of Alternaria alternata 1.0101 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.")