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Head Development Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Head Development Pathway and Crest Syndrome, Nervousness, Congenital Abnormality, Eye Abnormalities, Holoprosencephaly. The study of the Head Development Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Head Development Pathway has been researched in relation to Segmentation, Gastrulation, Cell Proliferation, Brain Development, Head Morphogenesis. The Head Development Pathway complements our catalog of research reagents including antibodies and ELISA kits against WNT, CHORDIN, BMP4, BTD, EGFR.

Top Research Reagents

We have 2477 products for the study of the Head Development Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Otx1 Antibody (PSH01-62)
NBP3-32682
Western Blot: Otx1 Antibody (PSH01-62) [NBP3-32682] - Western blot analysis of Otx1 on different lysates with Rabbit anti-Otx1 antibody (NBP3-32682) at 1/1,000 dilution.<br/><br/> Lane 1: 293T transfected with FLAG-tagged empty control cell lysate<br/> Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 32 kDa<br/> Observed band size: 32 kDa<br/><br/> Exposure time: 24 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32682) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.Immunohistochemistry: Otx1 Antibody (PSH01-62) [NBP3-32682] - Immunohistochemical analysis of paraffin-embedded rat eye tissue with Rabbit anti-Otx1 antibody (NBP3-32682) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32682) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

CYP4V2 Antibody
NBP3-38288
Immunocytochemistry/ Immunofluorescence: CYP4V2 Antibody [NBP3-38288] - Immunofluorescence analysis of NIH/3T3 cells using CYP4V2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Western Blot: CYP4V2 Antibody [NBP3-38288] - Western blot analysis of various lysates, using CYP4V2 Rabbit pAb at 1:900 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

Wnt-1 Antibody (10C8) - BSA Free
NBP1-51575
Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

2 Publications
SIX3 Antibody (1A2)
H00006496-M04
Western Blot: Six3 Antibody (1A2) [H00006496-M04] - Analysis of SIX3 expression in FHs 173 WE.Western Blot: Six3 Antibody (1A2) [H00006496-M04] - Analysis of SIX3 expression in 293 (Cat # L026V1).

Mouse Monoclonal
Species Human
Applications WB, ELISA

LIM1 Antibody (OTI2D5)
NBP2-01926
Western Blot: LIM1 Antibody (OTI2D5) [NBP2-01926] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIM1.Immunocytochemistry/Immunofluorescence: LIM1 Antibody (OTI2D5) [NBP2-01926] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

4 Publications
SS18L1 Antibody
NBP2-20486
Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

SSBP3 Antibody (3E6)
H00023648-M01
Sandwich ELISA: SSBP3 Antibody (3E6) [H00023648-M01] - Detection limit for recombinant GST tagged SSBP3 is approximately 0.3ng/ml as a capture antibody.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, S-ELISA

2 Publications
Otx2 Antibody [Unconjugated]
AF1979
Western blot shows lysates of IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Otx2 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human Otx2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1979) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow, upper panel; Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

     2 Reviews

178 Publications
HNF-3 beta/FoxA2 Antibody [Unconjugated]
AF2400
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF-3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (<a class=HNF-3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Goat Polyclonal
Species Human
Applications WB, IHC, ChIP

99 Publications
EGFR Antibody [Unconjugated]
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, Flow

30 Publications
Noggin [Unconjugated]
6057-NG
Recombinant human Noggin (Catalog # 6057-NG) has a molecular weight (MW) of 58.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation in recombinant human Noggin (25 µg/mL) from either R&D Systems (Lot 1, Lot 2; Catalog # 6057-NG) or from two separate competitors (Competitor 1, Competitor 2). Control cells were not incubated in Noggin (No Noggin). The cells were stained for the early ectoderm marker, Otx2, and the neuroectoderm marker, SOX1. (A) Representative images of SOX1 (green), Otx2 (red), and DAPI (blue) staining in embryonic stem cells differentiated with Noggin from R&D Systems or Noggin from Competitor 2. (B) SOX1+ clusters were quantified under each of the indicated culture conditions. Cells treated with R&D Systems Noggin showed an increase in SOX1+ cells compared to both untreated and competitor-treated cells. R&D Systems Noggin showed consistent differentiation across the lots tested. BG01V human embryonic stem cells are licensed from ViaCyte, Inc.


Species Human
Applications BA

208 Publications
Dkk-1 [Unconjugated]
5439-DK
Recombinant Human Dkk‑1 (Catalog # 5439-DK) inhibits Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 10.0-250 ng/mL.JOY6 human iPSCs were cultured in media containing Cultrex™ Stem Cell Qualified RGF BME (<a class=


Species Human
Applications BA

90 Publications
Sonic Hedgehog/Shh [Unconjugated]
464-SH
Recombinant Mouse Sonic Hedgehog/Shh (C25Il), N-Terminus (Catalog # 464-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The activity is more than 30-fold greater than the top competitor's Sonic Hedgehog.Recombinant Mouse Sonic Hedgehog/Shh (C25II), N-Terminus (Catalog # 464-SH) has a molecular weight (MW) of 20.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).


Species Mouse
Applications BA

69 Publications
FGF-8 [Unconjugated]
423-F8
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED<sub>50</sub> for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.


Species Human, Mouse
Applications BA

     1 Review

63 Publications
BMP-4 [Unconjugated]
314-BP
Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED<sub>50</sub> for this effect is 0.70-7.00 ng/mL.1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in gel are due to variable glycosylation.<p style=


Species Human
Applications BA, BA

525 Publications
SP8 Antibody
NBP2-49109
Immunocytochemistry/Immunofluorescence: SP8 Antibody [NBP2-49109] - Staining of human cell line PC-3 shows localization to nucleus. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SP8 Antibody [NBP2-49109] - Staining of human kidney shows no positivity in cells in tubules as expected.

Rabbit Polyclonal
Species Human, Mouse
Applications ICC/IF, IHC, IHC-P

2 Publications
Biotinidase/BTD Antibody
NBP2-97093
Western Blot: Biotinidase/BTD Antibody [NBP2-97093] - Anti-Biotinidase/BTD rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 48 kDaImmunohistochemistry-Paraffin: Biotinidase/BTD Antibody [NBP2-97093] - Immunochemical staining of human Biotinidase/BTD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).

Rabbit Polyclonal
Species Human
Applications WB, ELISA, IHC

Aquaporin-0 Antibody - BSA Free
NBP2-92773
Western Blot: Aquaporin-0 Antibody [NBP2-92773] - Analysis of extracts of various cell lines, using Aquaporin-0 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.Immunohistochemistry-Paraffin: Aquaporin-0 Antibody [NBP2-92773] - Rat brain using MIP antibody at dilution of 1:100 (40x lens).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P