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Defecation Rhythm Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Defecation Rhythm Pathway and Chronic Constipation, Constipation, Pain, Adenoma, Congenital Abnormality. The study of the Defecation Rhythm Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Defecation Rhythm Pathway has been researched in relation to Defecation, Rna Interference, Rhythmic Process, Regulation Of Defecation, Response To Osmotic Stress. The Defecation Rhythm Pathway complements our catalog of research reagents including antibodies and ELISA kits against ENAC, OLF-1, VAS, PSD-95, OSR.

Top Research Reagents

We have 868 products for the study of the Defecation Rhythm Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

H00005662-B01P
Western Blot: PSD Antibody [H00005662-B01P] - Analysis of PSD expression in rat brain.Immunocytochemistry/Immunofluorescence: PSD Antibody [H00005662-B01P] - Analysis of purified antibody to PSD on HeLa cell. (antibody concentration 10 ug/ml)

Mouse Polyclonal
Species Human, Rat
Applications WB, ICC/IF

NB110-81601
Western Blot: TRPM2 Antibody [NB110-81601] - Lane 1: CHO cells untransfected. Lane 2: CHO cell transfected with rat TRPM2 Lane 3: Mouse cortical neurons (cultured) Photo courtesy of Dr. Paco Herson, Oregon Health & Science University.Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-81601] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TRPM2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Mouse, Rat
Applications WB, ICC/IF, IHC

13 Publications
NBP1-85047
Western Blot: Tight Junction Protein 1 Antibody [NBP1-85047] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and TJP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:200 of Rabbit Anti-Human TJP1 Polyclonal Antibody (Catalog # NBP1-85047) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TJP1 at approximately 260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining in human testis and skeletal muscle tissues. Corresponding TJP1 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     5 Reviews

20 Publications
NBP1-88370
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Analysis in human skin and cerebral cortex tissues. Corresponding TYRP1 RNA-seq data are presented for the same tissues.Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in control (vector only transfected HEK293T lysate) and TYRP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

4 Publications
NBP2-02356
Western Blot: TPMT Antibody (2A2) [NBP2-02356] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT.Immunocytochemistry/Immunofluorescence: TPMT Antibody (2A2) [NBP2-02356] - Immunofluorescent staining of HepG2 cells using anti-TPMT mouse monoclonal antibody.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-02623
Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

1 Publication
NBP2-12906
Western Blot: TRPM7 Antibody (S74) [NBP2-12906] - Western Blot analysis of Human Cell lysates showing detection of TrpM7 protein using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: TRPM7 Antibody (S74) [NBP2-12906] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpM7 Monoclonal Antibody, Clone S74 (NBP2-12906). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpM7 Monoclonal Antibody (NBP2-12906) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpM7 Antibody (D) Composite.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-12913
Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-13304
Western Blot: SGSM3 Antibody [NBP2-13304] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4Immunocytochemistry/Immunofluorescence: SGSM3 Antibody [NBP2-13304] - Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-20383
Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

NB100-98864

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

3 Publications
AF5165
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Daudi human Burkitt's lymphoma cell line, and CH-1 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse EBF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5165) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Differential binding of transcription factors on EBV genomes in type I and type III latency.(A) ChIP-Seq tracks mapped to EBV genome in LCL (red) or Mutu I (blue) for EBF1 or RBP-j kappa  as indicated. EBNA2 ChIP-Seq for LCL (black). Y-axis represent raw read counts. (B) ChIP-qPCR for EBF1 or RBP-j kappa  in LCL (red) or Mutu I (blue) at various EBV genome regulatory regions as indicated and Actin genomic region as negative control. (C) Same as in B, except for isogenic EBV positive BL cells Kem III (red) or Kem I (blue). Asterisk indicates p < 0.05. (D) Western blot of Mutu I, LCL, Kem I, and Kem III probed with antibody to EBF1, RBP-j kappa , LMP1, and EBNA2, with cellular loading controls for GAPDH and H3K4me3, as indicated. (E) RT-qPCR for EBV type III gene expression in LCL or Mutu I (left panel) or Kem I and Kem III (right panel). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26752713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Human, Mouse
Applications WB

3 Publications
NBP2-46421
Western Blot: PISD Antibody (4G5) [NBP2-46421] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PISD .Immunohistochemistry: PISD Antibody (4G5) [NBP2-46421] - Analysis of Adenocarcinoma of Human ovary tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Mouse monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NBP2-49671
Immunocytochemistry/Immunofluorescence: TBP like protein TLP Antibody [NBP2-49671] - Staining of human cell line SK-MEL-30 shows localization to nucleus & nucleoli.Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP2-49671] - Staining in human testis and liver tissues using anti-TBPL1 antibody. Corresponding TBPL1 RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human
Applications ICC/IF, IHC, IHC-P

NB300-167
Immunohistochemistry: SHANK1 Antibody [NB300-167] - Synaptic distribution of Shank1/2 and somato-synaptic distribution of Shank3 in the mouse hippocampus, codistribution with VGLUT1, no codistribution with VGLUT2. 5x magnification of hippocampus. Immunofluorescence stainings of coronal sections from wild-type mice probed with Shank1-3 (white; green in merge) and VGLUT1 (white; red in merge) antibodies; green arrow points toward the intragranular mossy fibers where there is a prominent synaptic stain of Shank3; scale bar =300 um. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/Article/10.3389/fncel.2016.00106/abstract) licensed under a CC-BY license.Immunohistochemistry: SHANK1 Antibody [NB300-167] - Mouse retina using NB300-167.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

7 Publications