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Clathrin-mediated Endocytosis Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Clathrin-mediated Endocytosis Pathway and Neoplasms, Tissue Adhesions, Malignant Neoplasms, Nervousness, Cholera. The study of the Clathrin-mediated Endocytosis Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Clathrin-mediated Endocytosis Pathway has been researched in relation to Endocytosis, Transport, Macropinocytosis, Localization, Exocytosis. The Clathrin-mediated Endocytosis Pathway complements our catalog of research reagents including antibodies and ELISA kits against TRANSFERRIN RECEPTOR, EPSIN, AMPH, CAV1, DNM1.

Top Research Reagents

We have 2787 products for the study of the Clathrin-mediated Endocytosis Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB120-13253
Western Blot: Rab5a Antibody [NB120-13253] - Western blot analysis of Human Cell line lysates showing detection of Rab5aprotein using Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Rab5aPolyclonal Antibody (NB120-13253) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.Immunocytochemistry/Immunofluorescence: Rab5a Antibody [NB120-13253] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Rab5 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Nucleus. Magnification: 100x.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

17 Publications
NBP3-25690
Western Blot: AP180 Antibody (HL1657) - Azide and BSA Free [NBP3-25690] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SNAP91 antibody [HL1657] (NBP3-25690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: AP180 Antibody (HL1657) - Azide and BSA Free [NBP3-25690] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SNAP91 antibody [HL1657] (NBP3-25690) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

NB110-60491
Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate.  This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Simple Western, ELISA

NB100-615
Western Blot: Caveolin-1 Antibody (7C8) [NB100-615] - Detection of caveolin in 3T3 cell lysates (50 ug). Lanes 1 and 2: 1:4000.  Lanes 3 and 4: 1:1000. Detection by ECL: 5 minute exposure.Immunocytochemistry/Immunofluorescence: Caveolin-1 Antibody (7C8) [NB100-615] - Subcellular localization of mPARM-1 and hPARM-1 (full-length and mutant proteins). NIH/3T3 cells transiently expressing hPARM-1-GFP or deltaCT-GFP were fixed, immunostained for caveolin-1 (1:100, Novus Biologicals), and examined by confocal fluorescence microscopy. For hPARM-1-GFP-caveolin-1 co-localization, cells that  demonstrated cell membrane PARM-1 localization were chosen. All co-localizations were observed following merging images of GFP-tagged proteins with those of Golgi, endosomes, plasma membrane, alpha-tubulin or caveolin-1 labeling. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-84), licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     3 Reviews

37 Publications
H00010097-M01
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - ACTR2 monoclonal antibody (M01), clone 1B10-C4 Analysis of ACTR2 expression in Hela S3 NE.Immunohistochemistry-Paraffin: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Analysis of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, IHC

NBP1-87561
Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining in human cerebral cortex and tonsil tissues using anti-AMPH antibody. Corresponding AMPH RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Amphiphysin/AMPH Antibody [NBP1-87561] - Staining of human cerebral cortex, colon, kidney and tonsil using Anti-AMPH antibody NBP1-87561 (A) shows similar protein distribution across tissues to independent antibody NBP1-86033 (B).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

NBP2-04017
Western Blot: GTF3A Antibody [NBP2-04017] - Whole cell lysate (15 and 50 ug) from Jurkat cells.  Antibody used at 0.4 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB, IP

NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection. Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

1 Publication
AF2685
Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC

     1 Review

10 Publications
AF1230
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=    ERK2  was detected in immersion fixed paraffin-embedded sections of human breast  using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room  temperature followed by incubation with the Anti-Rabbit IgG  VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, KO

3 Publications
AF1443
Western blot shows lysates of mouse thymus tissue and mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat FABP4/A-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=FABP4 was detected in immersion fixed mouse mesenchymal stem cell-derived adipocytes using 10 µg/mL Goat Anti-Mouse/Rat FABP4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1443) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

Goat Polyclonal
Species Mouse, Rat
Applications WB, Simple Western, IHC

     2 Reviews

39 Publications
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, Flow

30 Publications
AF8480
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=Eps15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Eps15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8480) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # <a class=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, ICC

2914-HT


Species Human
Applications BA

51 Publications
236-EG
Recombinant Human EGF Protein (Catalog # 236-EG) has a molecular weight (MW) of 6.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED<sub>50</sub> for this effect is 20‑100 pg/mL.


Species Human
Applications BA

829 Publications
NBP2-47477
Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker  [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.  Lane 2: Human cell line RT-4.  Lane 3: Human cell line U-251MG.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

3 Publications
NB100-74359
Western Blot: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis was performed on whole cell extracts (30 ug lysate) of PC-3 (Lane1), A-431 (Lane2), Mouse Placenta (Lane3) and RAW 264.7 (Lane5). Immunocytochemistry/Immunofluorescence: AP2 alpha Antibody (3B5) [NB100-74359] - Analysis of AP2 using Anti-AP2 Monoclonal Antibody (3B5) shows staining in Hela Cells. AP2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing AP2 at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Mouse Monoclonal
Species Human, Mouse, Chicken
Applications WB, ICC/IF, IHC

     1 Review

3 Publications
NB300-204
Western Blot: HIP1 Antibody (1B11) [NB300-204] - HeLa whole cell extracts.Immunocytochemistry/Immunofluorescence: HIP1 Antibody (1B11) [NB300-204] - Human pluripotent embryonal carcinoma cells stained with HIP1 antibody. ICC/IF image submitted by a verified customer review.

Mouse Monoclonal
Species Human, Mouse, Bacteria
Applications WB, ICC/IF, IP

     1 Review

7 Publications