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![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0014.jpg "Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Mice with two different spontaneous mutant alleles of Sharpin show variations in onset of CPDM phenotype. However, the onset is much more rapid in Sharpincpdm-Dem mice as evidenced by antibody detection of cleaved CASPASE 9 (T) when compared to Sharpincpdm mutants (R) or WT mice (Q,S). . Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085666) licensed under a CC-BY license.")
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Autophagy-deficient mice showed increased activity of apoptosis and necroptosis. Increased the expression of Caspase-9 in 12-week-old Atg7deltapan mice. Caspase-9 quantitation and representative IF microphotographs of Atg7F/F (n=5) and Atg7deltapan (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (green) (1/1000, scale bar=50?um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0013.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Capase-9 in mouse transplanted lung tumor section using anti-Caspase-9 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56118-img0006.jpg)
![Immunohistochemistry: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis and altered inflammation in double-deficient Atg7deltapan-Rip3-/- mice. (c) Depletion of Rip3 and pancreatic Atg7 (Atg7deltapan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation and representative IF microphotographs of Atg7deltapan (n=5) and Atg7deltapan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) and Caspase-9 (red) (1/1000).. Image collected and cropped by CiteAb from the following publication (//www.nature.com/doifinder/10.1038/cddis.2017.313), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-NB100-56118-img0012.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for Active/Cleaved Caspase-9 expression at 1:2000. A and B. The pattern of Caspase-9 staining may vary between different types of neurons. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56118-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis of Dog Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a dog brain 2 hr after reperfusion injury. B) ection from a dog brain sham control (brain surgery but no injury). Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56118-img0008.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56118] - Analysis Human Brain sections stained for cleaved Caspase-9 expression using this antibody at 1:2000. A) Section from a patient 24 hr after head trauma. B) Control: section from a patient with no known neurological disease or head injury. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56118-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody [NB100-56118] - Exacerbated apoptosis & altered inflammation in double-deficient Atg7 delta pan-Rip3−/− mice. (a) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-3 in 12-week-old mice. Caspase-3 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-active caspase-3 ab2302, 1/50, scale bar=50 μm). (b) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic Bax in 12-week-old mice. Bax quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-3 (red) (anti-Bax (sc-526, 1/50). (c) Depletion of Rip3 & pancreatic Atg7 (Atg7 delta pan-Rip3d/d) increased pancreatic caspase-9 in 12-week-old mice. Caspase-9 quantitation & representative IF microphotographs of Atg7 delta pan (n=5) & Atg7 delta pan-Rip3d/d (n=5) pancreatic tissue stained for DAPI (blue) & Caspase-9 (red) (anti-caspase-9 NB100-56118, 1/1000). (d) Reduced infiltration inflammation of macrophages (F4/80, NBP2-12506, 1/75) & early T-lymphocytes (MPO, ab9535, 1/50) in double-deficient Atg7 delta pan-Rip3d/d (n=10) mice compared with pancreatic Atg7 delta pan (n=7). Data are mean±S.E.M. for the numbers of animals as indicated in the graph, *P<0.05, **P<0.01, NS = no significance Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28703808), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56118_rabbit-polyclonal-caspase-9-antibody-310202415533834.jpg)
![Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Western-Blot-NB500-209-img0006.jpg "Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - Western blot analysis of Jurkat (A) and HeLa (B) cell lysate using Caspase 9 (NB500-209) antibody at 1:500.")
![Immunocytochemistry/Immunofluorescence: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The Caspase 9 antibody was tested in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Immunocytochemistry-Immunofluorescence-NB500-209-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - IHC analysis of paraffin-embedded human breast carcinomausing Caspase 9 antibody at 1:25. Image from verified customer review.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Immunohistochemistry-Paraffin-NB500-209-img0008.jpg)
![Western Blot: Caspase 9 Antibody (LAP6 96-2-22) [NB500-209] - The pro and active forms of Caspase 9 detected in active 293 cell lysate (lane 2). Lane 1: inactive 293 cell lysate.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Western-Blot-NB500-209-img0002.jpg)
![Simple Western: Caspase-9 Antibody (LAP6 96-2-22) [NB500-209] - Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Staurosporine (STS) for 3 hours, loaded at 0.2 mg/mL. A specific band was detected for Caspase 9 at approximately 53 kDa (as indicated) using 20 ug/mL of Mouse Anti-Caspase 9 Monoclonal Antibody (Catalog # NB500-209). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-LAP6-96-2-22-Simple-Western-NB500-209-img0010.jpg)
![Western Blot: Caspase-9 Antibody [NB100-56119] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NB100-56119-img0003.jpg "Western Blot: Caspase-9 Antibody [NB100-56119] - Analysis of Caspase-9. Various tissue lysates were prepared from human autospsy material and normalized for total protein. Most tissues contained the ~50 kDa pro-Caspase-9 protein. Cleaved Caspase-9 was identified in brain cortex and peripheral nerve tissue samples. Pro-C9: recombinant human pro-Caspase-9 (full-length) protein. PRO: pro-Caspase-9. L: large subunit of cleaved Caspase-9. S: small subunit of cleaved Caspase-9.")
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NB100-56119] - Mouse brain tissue sections at 1:2000. Hematoxylin-eosin counterstain. A) Brain striatum, B) Brain stem motor neuron.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-NB100-56119-img0002.jpg)
![Simple Western: Caspase-9 Antibody [NB100-56119] - Lane view shows a specific band for Caspase 9 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Simple-Western-NB100-56119-img0004.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0008.jpg "Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - ICC staining of Caspase-9 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primar](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0007.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0005.jpg)
![Western Blot: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Western-Blot-NBP2-67362-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Staining Caspase-9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunocytochemistry-Immunofluorescence-NBP2-67362-img0003.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody (SZ29-01) - (Pro and Active) [NBP2-67362] - Analysis of paraffin-embedded human tonsil tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-SZ29-01-Pro-and-Active-Immunohistochemistry-Paraffin-NBP2-67362-img0004.jpg)
![Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0015.jpg "Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Mouse Cell Line. Loading: 15 ug of 3T3/NIH cell lysate.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Caspase-9 Antibody [NBP1-76961] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, NBP1-76470 (1 ug/mL), Caspase 9, NBP1-76961 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0013.jpg)
![Western Blot: Caspase-9 Antibody [NBP1-76961] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane.Antibodies: : Caspase 9, NBP1-76961 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-76961-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-76961] - Staining of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76961-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 5 Immunofluorescence Validation of Caspase 9 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with at 5 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-271120241937769.gif)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 4 Immunofluorescence Validation of Caspase 9 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-271120242092046.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 6 Immunocytochemistry Validation of Caspase 9 in HeLa CellsImmunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody at 5 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-2711202420285838.gif)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 5 Immunofluorescence Validation of Caspase 9 in HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with at 5 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-2711202419541536.gif)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 4 Immunofluorescence Validation of Caspase 9 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-2711202420285814.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-9 Antibody - BSA Free [NBP1-76961] - Figure 6 Immunocytochemistry Validation of Caspase 9 in HeLa CellsImmunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody at 5 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76961_rabbit-polyclonal-caspase-9-antibody-2711202420141918.gif)
![Western Blot: Caspase-9 Antibody (2-22) [NBP1-19231] - Analysis on human HeLa and HeLa induced cell lysates.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-2-22-Western-Blot-NBP1-19231-img0012.jpg "Western Blot: Caspase-9 Antibody (2-22) [NBP1-19231] - Analysis on human HeLa and HeLa induced cell lysates.")
![Immunohistochemistry: Caspase-9 Antibody (2-22) [NBP1-19231] - Human uterus, vessels tissue stained with Caspase-9, mAb (2-22) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-2-22-Immunohistochemistry-NBP1-19231-img0011.jpg)
![Immunohistochemistry: Caspase-9 Antibody (2-22) [NBP1-19231] - Human spleen tissue stained with Caspase-9, mAb (2-22) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-2-22-Immunohistochemistry-NBP1-19231-img0006.jpg)
![Immunohistochemistry: Caspase-9 Antibody (2-22) [NBP1-19231] - Human heart tissue stained with Caspase-9, mAb (2-22) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-2-22-Immunohistochemistry-NBP1-19231-img0010.jpg)
![Western Blot: Caspase-9 [p Ser196] Antibody [NB100-56122] - Analysis of human Jurkat cells were left untreated or were staurosporine-treated, lysed and probed with Caspase- 9 antibody at 1:1000.](http://images.novusbio.com/fullsize/Caspase-9-[p-Ser196]-Antibody-Western-Blot-NB100-56122-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-9 [p Ser196] Antibody [NB100-56122] - Analysis of human Jurkat cells were left untreated or were staurosporine-treated, lysed and probed with Caspase- 9 antibody at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-9 [p Ser196] Antibody - Unpurified [NB100-56122] - Caspase-9 [p Ser196] Antibody [NB100-56122] - Human colon cancer using Caspase-9 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Caspase 9 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Caspase-9-[p-Ser196]-Antibody-Immunohistochemistry-Paraffin-NB100-56122-img0005.jpg)
![Western Blot: Caspase-9 Antibody [NB100-56121] - Analysis of Caspase-9 in human HeLa cell lysate at 1:1000.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NB100-56121-img0005.jpg "Western Blot: Caspase-9 Antibody [NB100-56121] - Analysis of Caspase-9 in human HeLa cell lysate at 1:1000.")
![Immunohistochemistry-Paraffin: Caspase-9 Antibody - Unpurified [NB100-56121] - Caspase-9 Antibody [NB100-56121] - analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using Caspase-9 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Caspase 9 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NB100-56121-img0006.jpg)
![Western Blot: Caspase-9 Antibody [NBP1-90142] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-90142-img0010.jpg "Western Blot: Caspase-9 Antibody [NBP1-90142] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody [NBP1-90142] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90142-img0009.jpg)
![Immunohistochemistry-Paraffin: Caspase-9 Antibody [NBP1-90142] - Staining of human rectum shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Immunohistochemistry-Paraffin-NBP1-90142-img0002.jpg)
![Western Blot: Caspase-9 Antibody [NBP1-90142] - Analysis in human cell line HeLa.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-Western-Blot-NBP1-90142-img0007.jpg)
![Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Western-Blot-NBP3-15590-img0005.jpg "Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Immunocytochemistry-Immunofluorescence-NBP3-15590-img0003.jpg)
![Western Blot: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Analysis of extracts of various cell lines, using Caspase-9 antibody at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Western-Blot-NBP3-15590-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (4E5G9) [NBP3-15590] - Immunofluorescence analysis of C6 cells using [KO Validated] Caspase-9 Rabbit mAb (NBP3-15590) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Caspase-9-Antibody-4E5G9-Immunocytochemistry-Immunofluorescence-NBP3-15590-img0002.jpg)
![Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241140940.jpg "Western Blot: Caspase-9 Antibody (2D5) [NBP3-26697] - Positive Western Blot detected in: HL-60 whole cell lysate, LO2 whole cell lysate. All lanes: Caspase-9 Antibody at 1.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 31, 18, 37 KDa Observed band size: 47 KDa")
![Immunocytochemistry/Immunofluorescence: Caspase-9 Antibody (2D5) [NBP3-26697] - Staining of HepG2 cells with Caspase-9 Antibody (2D5) at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-28220241323614.jpg)
![Flow Cytometry: Caspase-9 Antibody (2D5) [NBP3-26697] - Overlay histogram showing K562 cells stained with Caspase-9 Antibody (2D5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26697_rabbit-caspase-9-mab-2d5-282202414183649.jpg)