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![Western Blot: TRPM8 Antibody [NBP1-97311] - The results of co-immunoprecipitation (CoIP) confirmed interaction between NFkB and TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 and NFkB. Data are shown as the mean +/- S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Knockdown-Validated-NBP1-97311-img0007.jpg "<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: TRPM8 Antibody [NBP1-97311] - The results of co-immunoprecipitation (CoIP) confirmed interaction between NFkB and TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 and NFkB. Data are shown as the mean +/- S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - Co-localization of TRPM8 and TNFa in the cytoplasm (cold condition and 500 nM menthol). Data are shown as the mean +/- S.D. from three experiments. **P < 0.01.Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97311-img0008.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: TRPM8 Antibody [NBP1-97311] - Brain cooling means putting the anesthetized mouse brain on the artificial ice. Normal means the room temperature (25C). Data are shown as the mean +/- S.D. from five mice in each group. #P < 0.05; ##P < 0.01, v.s. the normal control. *P < 0.05; v.s. the CIR. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP1-97311-img0006.jpg)
![Immunohistochemistry: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunohistochemistry-NBP1-97311-img0005.jpg)
![Western Blot: TRPM8 Antibody [NBP1-97311] - Analysis of TRPM8 in PC12 cell lysate.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP1-97311-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP1-97311] - TRPM8 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97311-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Confocal imaging of TRPM8, NF kappa B & TNF alpha expression in PC12 cells.(A) The expression of TRPM8, NF kappa B & TNF alpha in wild type cells & Trpm8 knockdown cells at 37 °C & 4 °C. KD signifies Trpm8 knockdown cells. (B) In wild type cells, TRPM8 was upregulated & NF kappa B & TNF alpha were downregulated under cold conditions. (C) In KD cells, TRPM8 showed weak expression & NF kappa B & TNF alpha expression levels were increased. (D) Co-localization of TRPM8 & TNF alpha in the cytoplasm (cold condition & 500 nM menthol). Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-31020241536377.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappa B & TNF alpha in PC12 cells with Trpm8 knockdown under cold conditions (4 °C).(A) Construction of a Trpm8 (Dylight 649) knockdown stable cell line. WT represents wild type cells. KD signifies the Trpm8 knockdown cells. (B,C) Protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . NS: no significance. Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415291969.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Alteration of core temperature & the expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha in mouse brains under cold conditions.(A) Core body temperature under cold conditions (4 °C). (B) The mRNA expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . (C,D) The protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . Data are shown as the mean ± S.D. from 12 mice in each group. ##v.s. the control (zero hour), P < 0.01; #P < 0.05. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415392570.jpg)
![Immunocytochemistry/ Immunofluorescence: TRPM8 Antibody - BSA Free [NBP1-97311] - Co-localization of TRPM8 & NF kappa B in PC12 cells under cold conditions.(A) Immunofluorescence assay image of TRPM8 & NF kappa B in the cytoplasm. WT represents wild type cells. KD represents Trpm8 knockdown cells. (B) The results of co-immunoprecipitation (CoIP) of endogenous TRPM8 & NF kappa B using NF kappa B antibodies. Western blot analysis was carried out to detect TRPM8 & NF kappa B. (C) Reverse CoIP confirmed interaction between NF kappa B & TRPM8. CoIPs were also performed with TRPM8 antibodies. Western blot analysis was carried out by TRPM8 & NF kappa B. Data are shown as the mean ± S.D. from three experiments. *P < 0.05; **P < 0.01. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-31020241539721.jpg)
![Western Blot: TRPM8 Antibody - BSA Free [NBP1-97311] - Expression of TRPM8, TRPA1, NF kappa B & TNF alpha in PC12 cells under cold conditions.(A) Intracellular Ca2+ in the cells under cold conditions (4 °C). The Ca2+ concentration in the cytoplasm at 4 °C is higher than that at 37 °C. (B) The mRNA expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . (C,D) The protein expression levels of TRPM8, TRPA1, NF kappa B & TNF alpha . Data are shown as the mean ± S.D. from three experiments. #P < 0.05; ##P < 0.01, v.s. the control (zero time). Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/srep45155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97311_rabbit-polyclonal-trpm8-antibody-310202415304224.jpg)
![Western Blot: TRPM8 Antibody [NB200-145] - Analysis of TRPM8 in transfected COS cells using NB200-145 at 1:750 and incubated overnight at 4C. Secondary rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NB200-145-img0001.jpg "Western Blot: TRPM8 Antibody [NB200-145] - Analysis of TRPM8 in transfected COS cells using NB200-145 at 1:750 and incubated overnight at 4C. Secondary rabbit HRP antibody was incubated 1 hour at RT followed by ECL detection.")
![Immunohistochemistry: TRPM8 Antibody [NB200-145] - Prostate cancer: Formalin-Fixed Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunohistochemistry-NB200-145-img0002.jpg)
![Western Blot: TRPM8 Antibody (M572) [NBP3-23238] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (NBP3-23237) (lane 1) or clone M572 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23238_mouse-trpm8-mab-m572-199202312523649.jpg "Western Blot: TRPM8 Antibody (M572) [NBP3-23238] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (NBP3-23237) (lane 1) or clone M572 (lane 2).")
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody (M572) [NBP3-23238] - Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M572). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23238_mouse-trpm8-mab-m572-19920231410144.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP3-23239] - Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (NBP3-23239). The antibody was detected using goat anti-rabbit DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23239_rabbit-trpm8-pab-1992023141011.jpg "Immunocytochemistry/Immunofluorescence: TRPM8 Antibody [NBP3-23239] - Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (NBP3-23239). The antibody was detected using goat anti-rabbit DyLight(R) 594.")
![Western Blot: TRPM8 Antibody [NBP3-23239] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with rabbit polyclonal anti-TRPM8 (extracellular region) without peptide blocking (lane 1) or with peptide blocking (lane 2).](http://images.novusbio.com/fullsize/nbp3-23239_rabbit-trpm8-pab-1992023191542.jpg)
![Western Blot: TRPM8 Antibody (M571) [NBP3-23237] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).](http://images.novusbio.com/fullsize/nbp3-23237_mouse-trpm8-mab-m571-1192023166535.jpg "Western Blot: TRPM8 Antibody (M571) [NBP3-23237] - Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).")
![Immunocytochemistry/Immunofluorescence: TRPM8 Antibody (M571) [NBP3-23237] - Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M571). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23237_mouse-trpm8-mab-m571-19920231410132.jpg)
![Western Blot: TRPM8 Antibody (JE32-58) [NBP3-33062] - Western blot analysis of TRPM8 on different lysates with Rabbit anti-TRPM8 antibody (NBP3-33062) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 128 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33062) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33062_rabbit-trpm8-mab-je32-58-288202412493448.jpg "Western Blot: TRPM8 Antibody (JE32-58) [NBP3-33062] - Western blot analysis of TRPM8 on different lysates with Rabbit anti-TRPM8 antibody (NBP3-33062) at 1/1,000 dilution. Lane 1: LNCaP cell lysate Lane 2: MCF7 cell lysate Lane 3: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 128 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33062) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TRPM8 Antibody (JE32-58) [NBP3-33062] - Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-TRPM8 antibody (NBP3-33062) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33062) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33062_rabbit-trpm8-mab-je32-58-298202414262669.jpg)
![Western Blot: TRPM8 Antibody (SR1110) [NBP3-21832] - Western blot analysis using NBP3-21832 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21832_rabbit-trpm8-mab-sr1110-5720231526110.jpg "Western Blot: TRPM8 Antibody (SR1110) [NBP3-21832] - Western blot analysis using NBP3-21832 in A549 cell lysate.")
![Immunohistochemistry: TRPM8 Antibody [NBP3-10364] - Immunohistochemical analysis of prostate tissue at an antibody concentration of 5ug/ml.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Immunohistochemistry-NBP3-10364-img0001.jpg "Immunohistochemistry: TRPM8 Antibody [NBP3-10364] - Immunohistochemical analysis of prostate tissue at an antibody concentration of 5ug/ml.")
![Western Blot: TRPM8 Antibody [NBP2-95133] - Analysis of extracts of various cell lines, using TRPM8 . Exposure time: 60s.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP2-95133-img0001.jpg "Western Blot: TRPM8 Antibody [NBP2-95133] - Analysis of extracts of various cell lines, using TRPM8 . Exposure time: 60s.")
![Western Blot: TRPM8 Antibody [NBP1-33165] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/TRPM8-Antibody-Western-Blot-NBP1-33165-img0003.jpg "Western Blot: TRPM8 Antibody [NBP1-33165] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE, antibody diluted at 1:500.")