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![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining in human adrenal gland and kidney tissues using anti-PENK antibody. Corresponding PENK RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining in human adrenal gland and kidney tissues using anti-PENK antibody. Corresponding PENK RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Met-enkephalin Antibody [NBP1-90944] - Analysis in control (vector only transfected HEK293T lysate) and PENK over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Western-Blot-NBP1-90944-img0011.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human adrenal gland shows strong cytoplasmic positivity in medullary cells.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0008.jpg)
![Immunohistochemistry-Paraffin: Met-enkephalin Antibody [NBP1-90944] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/Met-enkephalin-Antibody-Immunohistochemistry-Paraffin-NBP1-90944-img0009.jpg)
![Western Blot: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Analysis of Prodynorphin/PDYN expression in transfected 293T cell line by Prodynorphin/PDYN monoclonal antibody (M01), clone 2E12.Lane 1: Prodynorphin/PDYN transfected lysate(28.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-2E12-Western-Blot-H00005173-M01-img0003.jpg "Western Blot: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Analysis of Prodynorphin/PDYN expression in transfected 293T cell line by Prodynorphin/PDYN monoclonal antibody (M01), clone 2E12.Lane 1: Prodynorphin/PDYN transfected lysate(28.4 KDa).Lane 2: Non-transfected lysate.")
![Immunoprecipitation: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Analysis of Prodynorphin/PDYN transfected lysate using anti-Prodynorphin/PDYN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with Prodynorphin/PDYN MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-2E12-Immunoprecipitation-H00005173-M01-img0001.jpg)
![Sandwich ELISA: Prodynorphin/PDYN Antibody (2E12) [H00005173-M01] - Detection limit for recombinant GST tagged Prodynorphin/PDYN is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-2E12-Sandwich-ELISA-H00005173-M01-img0002.jpg)
![Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP1-31768] - Human colon carcinoma, using Prodynorphin/PDYN antibody at 1:250 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP1-31768-img0002.jpg "Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP1-31768] - Human colon carcinoma, using Prodynorphin/PDYN antibody at 1:250 dilution. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Prodynorphin/PDYN Antibody [NBP1-31768] - Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ProDynorphin antibody [N1C2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-31768_rabbit-polyclonal-prodynorphin-pdyn-antibody-western-blot-1422025125546..png)
![Western Blot: Prodynorphin/PDYN Antibody [NBP1-31768] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ProDynorphin antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-31768_rabbit-polyclonal-prodynorphin-pdyn-antibody-western-blot-142202512576..png)
![Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38195_rabbit-prodynorphin-pdyn-pab-91220241515281.jpg "Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38195_rabbit-prodynorphin-pdyn-pab-912202414213746.jpg)
![Western Blot: Prodynorphin/PDYN Antibody [NBP3-38195] - Western blot analysis of various lysates using Prodynorphin/PDYN Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38195_rabbit-prodynorphin-pdyn-pab-91220241516182.jpg)
![Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38195_rabbit-prodynorphin-pdyn-pab-912202415161848.jpg)
![Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38195_rabbit-prodynorphin-pdyn-pab-91220241519599.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252820.jpg "Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Human prostate using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: Proenkephalin Antibody [NBP3-38239] - Immunofluorescence analysis of paraffin-embedded Human adrenal gland using Proenkephalin Rabbit pAb at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241252847.jpg)
![Immunohistochemistry: Proenkephalin Antibody [NBP3-38239] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Proenkephalin Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-101220241224152.jpg)
![Western Blot: Proenkephalin Antibody [NBP3-38239] - Western blot analysis of lysates from THP-1 cells, using Proenkephalin Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.](http://images.novusbio.com/fullsize/nbp3-38239_rabbit-proenkephalin-pab-10122024126238.jpg)
![Western Blot: Proenkephalin Antibody (9E7) [H00005179-M04] - Analysis of PENK expression in transfected 293T cell line by PENK monoclonal antibody (M04), clone 9E7. Lane 1: PENK transfected lysate (Predicted MW: 29.48 kDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-9E7-Western-Blot-H00005179-M04-img0002.jpg "Western Blot: Proenkephalin Antibody (9E7) [H00005179-M04] - Analysis of PENK expression in transfected 293T cell line by PENK monoclonal antibody (M04), clone 9E7. Lane 1: PENK transfected lysate (Predicted MW: 29.48 kDa). Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Proenkephalin Antibody (9E7) [H00005179-M04] - Detection limit for recombinant GST tagged PENK is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-9E7-Sandwich-ELISA-H00005179-M04-img0001.jpg)
![Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Analysis in human cerebral cortex and liver tissues using NBP2-49081 antibody. Corresponding Prodynorphin/PDYN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP2-49081-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Analysis in human cerebral cortex and liver tissues using NBP2-49081 antibody. Corresponding Prodynorphin/PDYN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP2-49081-img0003.jpg)
![Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP2-49081-img0004.jpg)
![Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP2-49081-img0005.jpg)
![Immunohistochemistry-Paraffin: Prodynorphin/PDYN Antibody [NBP2-49081] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Immunohistochemistry-Paraffin-NBP2-49081-img0006.jpg)
![Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Immunohistochemistry-NBP1-20963-img0003.jpg "Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.")
![Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.](http://images.novusbio.com/fullsize/Proenkephalin-Antibody-Immunohistochemistry-NBP1-20963-img0001.jpg)
![Western Blot: Prodynorphin/PDYN Antibody [NBP1-91500] - Titration: 0.2-1 ug/ml, Positive Control: 293T cell lysate.](http://images.novusbio.com/fullsize/Dynorphin-Antibody-Western-Blot-NBP1-91500-img0003.jpg "Western Blot: Prodynorphin/PDYN Antibody [NBP1-91500] - Titration: 0.2-1 ug/ml, Positive Control: 293T cell lysate.")
![SDS-Page: Recombinant Human Dynorphin Protein [H00005173-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005173-Q01-1.jpg "SDS-Page: Recombinant Human Dynorphin Protein [H00005173-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")