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![Western Blot: DKC1 Antibody [NBP1-85156] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/DKC1-Antibody-Western-Blot-NBP1-85156-img0006.jpg "Western Blot: DKC1 Antibody [NBP1-85156] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human cell line A-431 Lane 5: Human liver tissue Lane 6: Human tonsil tissue")
![Immunohistochemistry-Paraffin: DKC1 Antibody [NBP1-85156] - Staining of human cerebral cortex shows strong nucleolar positivity in neuronal cells.](http://images.novusbio.com/fullsize/DKC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85156-img0001.jpg)
![Western Blot: DKC1 Antibody (2W2V6) [NBP3-16405] - Western blot analysis of extracts of PC-3 cells, using DKC1 Rabbit mAb (NBP3-16405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/DKC1-Antibody-2W2V6-Western-Blot-NBP3-16405-img0004.jpg "Western Blot: DKC1 Antibody (2W2V6) [NBP3-16405] - Western blot analysis of extracts of PC-3 cells, using DKC1 Rabbit mAb (NBP3-16405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: DKC1 Antibody (2W2V6) [NBP3-16405] - Immunohistochemistry of paraffin-embedded mouse testis using DKC1 Rabbit mAb (NBP3-16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DKC1-Antibody-2W2V6-Immunohistochemistry-Paraffin-NBP3-16405-img0003.jpg)
![Western Blot: DKC1 Antibody (2W2V6) [NBP3-16405] - Western blot analysis of extracts of various cell lines, using DKC1 Rabbit mAb (NBP3-16405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DKC1-Antibody-2W2V6-Western-Blot-NBP3-16405-img0005.jpg)
![Immunohistochemistry-Paraffin: DKC1 Antibody (2W2V6) [NBP3-16405] - Immunohistochemistry of paraffin-embedded rat brain using DKC1 Rabbit mAb (NBP3-16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DKC1-Antibody-2W2V6-Immunohistochemistry-Paraffin-NBP3-16405-img0001.jpg)
![Immunohistochemistry-Paraffin: DKC1 Antibody (2W2V6) [NBP3-16405] - Immunohistochemistry of paraffin-embedded human colon using DKC1 Rabbit mAb (NBP3-16405) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/DKC1-Antibody-2W2V6-Immunohistochemistry-Paraffin-NBP3-16405-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: DKC1 Antibody [NBP3-35287] - Immunofluorescence analysis of U-2 OS cells using DKC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35287_rabbit-dkc1-pab-10122024125283.jpg "Immunocytochemistry/ Immunofluorescence: DKC1 Antibody [NBP3-35287] - Immunofluorescence analysis of U-2 OS cells using DKC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: DKC1 Antibody [NBP3-35287] - Immunofluorescence analysis of NIH-3T3 cells using DKC1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35287_rabbit-dkc1-pab-10122024121049.jpg)
![Western Blot: DKC1 Antibody [NBP3-35287] - Western blot analysis of various lysates using DKC1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35287_rabbit-dkc1-pab-1012202411555912.jpg)
![Immunohistochemistry: DKC1 Antibody [NBP3-35287] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using DKC1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35287_rabbit-dkc1-pab-1012202411531091.jpg)
![Western Blot: DKC1 Antibody [NBP2-55221] - Analysis in human cell line A-431.](http://images.novusbio.com/fullsize/DKC1-Antibody-Western-Blot-NBP2-55221-img0004.jpg "Western Blot: DKC1 Antibody [NBP2-55221] - Analysis in human cell line A-431.")
![Immunocytochemistry/Immunofluorescence: DKC1 Antibody [NBP2-55221] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli fibrillar center.](http://images.novusbio.com/fullsize/DKC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55221-img0003.jpg)
![Western Blot: DKC1 Antibody [NBP2-55221] - Analysis in human cell line RT-4, human cell line U-251 MG and human cell line A-431.](http://images.novusbio.com/fullsize/DKC1-Antibody-Western-Blot-NBP2-55221-img0002.jpg)
![Western Blot: DKC1 Antibody (S02-2D8) [NBP3-14925] - Western blot detection of DKC1 in K562, C6, 3T3, Hela cell lysates using DKC1 Rabbit mAb (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.](http://images.novusbio.com/fullsize/DKC1-Antibody-S02-2D8-Western-Blot-NBP3-14925-img0001.jpg "Western Blot: DKC1 Antibody (S02-2D8) [NBP3-14925] - Western blot detection of DKC1 in K562, C6, 3T3, Hela cell lysates using DKC1 Rabbit mAb (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.")
![Immunohistochemistry-Paraffin: DKC1 Antibody (S02-2D8) [NBP3-14925] - Immunohistochemistry of DKC1 in paraffin-embedded Human breast cancer tissue using DKC1 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/DKC1-Antibody-S02-2D8-Immunohistochemistry-Paraffin-NBP3-14925-img0002.jpg)
![Immunohistochemistry-Paraffin: DKC1 Antibody [NBP1-85155] - Staining of human gall bladder shows distinct nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/DKC1-Antibody-Immunohistochemistry-Paraffin-NBP1-85155-img0002.jpg "Immunohistochemistry-Paraffin: DKC1 Antibody [NBP1-85155] - Staining of human gall bladder shows distinct nuclear positivity in glandular cells.")
![SDS-Page: Recombinant Human DKC1 Protein [H00001736-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001736-Q01-1.jpg "SDS-Page: Recombinant Human DKC1 Protein [H00001736-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: DKC1 Antibody [NBP1-41064] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-DKC1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. DKC1 was also immunoprecipitated by rabbit anti-DKC1 antibody NBP!-41065, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/DKC1-Antibody-Western-Blot-NBP1-41064-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: DKC1 Antibody [NBP1-41064] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-DKC1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. DKC1 was also immunoprecipitated by rabbit anti-DKC1 antibody NBP!-41065, which recognizes a downstream epitope.")
![Western Blot: DKC1 Overexpression Lysate (Adult Normal) [NBL1-09897] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DKC1.](http://images.novusbio.com/fullsize/DKC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09897-img0002.jpg "Western Blot: DKC1 Overexpression Lysate (Adult Normal) [NBL1-09897] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DKC1.")
![Western Blot: DKC1 Overexpression Lysate (Adult Normal) [NBP2-09647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DKC1.](http://images.novusbio.com/fullsize/DKC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09647-img0001.jpg "Western Blot: DKC1 Overexpression Lysate (Adult Normal) [NBP2-09647] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DKC1.")