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![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining in human heart muscle and skin tissues using anti-CASQ2 antibody. Corresponding CASQ2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining in human heart muscle and skin tissues using anti-CASQ2 antibody. Corresponding CASQ2 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining of human heart muscle, lymph node, skeletal muscle and skin using Anti-CASQ2 antibody NBP1-87304 (A) shows similar protein distribution across tissues to independent antibody NBP2-33747 (B).](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0010.jpg)
![Western Blot: Calsequestrin 2 Antibody [NBP1-87304] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-93](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Western-Blot-NBP1-87304-img0005.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining of human skeletal muscle.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0012.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0007.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0008.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-87304] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-87304-img0011.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human skeletal muscle.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0006.jpg "Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human skeletal muscle.")
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0001.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human heart muscle shows high expression.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0002.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining in human heart muscle and skin tissues using anti-CASQ2 antibody. Corresponding CASQ2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human heart muscle, lymph node, skeletal muscle and skin using Anti-CASQ2 antibody NBP2-33747 (A) shows similar protein distribution across tissues to independent antibody NBP1-87304 (B).](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0005.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP2-33747] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-33747-img0007.jpg)
![Western Blot: Calsequestrin 2 Antibody [NBP1-32594] - A. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE Calsequestrin 2 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Western-Blot-NBP1-32594-img0007.jpg "Western Blot: Calsequestrin 2 Antibody [NBP1-32594] - A. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE Calsequestrin 2 antibody dilution: 1:1000")
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-32594] - Paraffin-embedded mouse muscle Calsequestrin-2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32594-img0008.jpg)
![Western Blot: Calsequestrin 2 Antibody [NBP1-32594] - Sample(30 ug whole cell lysate)A:H129910% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Western-Blot-NBP1-32594-img0004.jpg)
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody [NBP1-32594] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32594-img0003.jpg)
![Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-298202414333685.jpg "Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32102) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32102, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-298202414262618.jpg)
![Western Blot: Calsequestrin 2 Antibody (PSH03-16) [NBP3-32102] - Western blot analysis of Calsequestrin 2 on different lysates with Rabbit anti-Calsequestrin 2 antibody (NBP3-32102) at 1/10,000 dilution.Lane 1: Mouse skeletal muscle tissue lysate (15 µg/Lane)Lane 2: Mouse heart tissue lysate (15 µg/Lane)Lane 3: Mouse brain tissue lysate (negative) (15 µg/Lane)Lane 4: Rat skeletal muscle tissue lysate (15 µg/Lane)Lane 5: Rat heart tissue lysate (15 µg/Lane)Lane 6: Rat brain tissue lysate (negative) (15 µg/Lane)Predicted band size: 46 kDaObserved band size: 55 kDaExposure time: 1 minute 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32102_rabbit-calsequestrin-2-mab-psh03-16-3920248453541.jpg)
![Western Blot: Calsequestrin 2 Antibody (1B6) [H00000845-M01] - Analysis of CASQ2 expression in transfected 293T cell line by CASQ2 monoclonal antibody (M01), clone 1B6.Lane 1: CASQ2 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-1B6-Western-Blot-H00000845-M01-img0003.jpg "Western Blot: Calsequestrin 2 Antibody (1B6) [H00000845-M01] - Analysis of CASQ2 expression in transfected 293T cell line by CASQ2 monoclonal antibody (M01), clone 1B6.Lane 1: CASQ2 transfected lysate(46.4 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: Calsequestrin 2 Antibody (1B6) [H00000845-M01] - Detection limit for recombinant GST tagged CASQ2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-1B6-Sandwich-ELISA-H00000845-M01-img0002.jpg)
![Western Blot: Calsequestrin 2 Antibody (4E10) [NBP1-74043] - The extracts of Mouse muscle (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CASQ2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-4E10-Western-Blot-NBP1-74043-img0001.jpg "Western Blot: Calsequestrin 2 Antibody (4E10) [NBP1-74043] - The extracts of Mouse muscle (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CASQ2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody (1C6) [H00000845-M07] - Analysis of monoclonal antibody to CASQ2 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Calsequestrin-2-Antibody-1C6-Immunohistochemistry-Paraffin-H00000845-M07-img0002.jpg "Immunohistochemistry-Paraffin: Calsequestrin 2 Antibody (1C6) [H00000845-M07] - Analysis of monoclonal antibody to CASQ2 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml")
![Western Blot: Calsequestrin 2 Overexpression Lysate (Adult Normal) [NBL1-08720] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calsequestrin 2.](http://images.novusbio.com/fullsize/Calsequestrin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08720-img0002.jpg "Western Blot: Calsequestrin 2 Overexpression Lysate (Adult Normal) [NBL1-08720] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Calsequestrin 2.")
![SDS-Page: Calsequestrin 2 Protein [NBP1-41229] - Calsequestrin-2, 48.4 kDa (417aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Calsequestrin-2-Protein-SDS-Page-NBP1-41229-img0001.jpg "SDS-Page: Calsequestrin 2 Protein [NBP1-41229] - Calsequestrin-2, 48.4 kDa (417aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")