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Segmental Glomerulosclerosis: Disease Bioinformatics

Research of Segmental Glomerulosclerosis has been linked to Glomerulosclerosis (disorder), Focal Glomerulosclerosis, Kidney Diseases, Proteinuria Of Undiagnosed Cause, Nephrotic Syndrome. The study of Segmental Glomerulosclerosis has been mentioned in research publications which can be found using our bioinformatics tool below. Researched pathways related to Segmental Glomerulosclerosis include Pathogenesis, Excretion, Glomerular Filtration, Cell Proliferation, Localization. These pathways complement our catalog of research reagents for the study of Segmental Glomerulosclerosis including antibodies and ELISA kits against MGN, ACTN4, AGT, ALB, C3.

Top Research Reagents

We have 4381 products for the study of Segmental Glomerulosclerosis that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Carbonic Anhydrase IX/CA9 Antibody - BSA Free
NB100-417
CA9 expressed in hypoxic regions as assessed by pimonidazole staining (A), but also observed in non-pimonidazole areas (B). Most of CA9 expressed in pimonidazole positive regions (C). Proliferation (BrdUrd) & apoptosis (caspase-3) in relation to CA9 expression shown in figure D & E. Red, CA9; Green, pimonidazole (A-B), BrdUrd (D) or caspase-3 (E); Yellow, overlap of CA9 (red) & pimonidazole (green); Light blue, vessels. Magnification 100x. Scale bars represent 100 um. Closed circles represent CA9 expression in pimonidazole positive regions; open circles represent CA9 expression in pimonidazole -ve regions. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0108068), licensed under a CC-BY license.Formalin-fixed paraffin-embedded tissue sections of human stomach were probed for Carbonic Anhydrase IX/CA9 mRNA (ACD RNAScope Probe, catalog # 559348; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using Rabbit Polyclonal (Novus catalog # NB100-417) at 1:1000 dilution with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     7 Reviews

279 Publications
KRAS Antibody (3B10-2F2)
H00003845-M01
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western Blot analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.<br><br>Lane 1: KRAS transfected lysate(21 KDa).<br>Lane 2: Non-transfected lysate.<br>

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

     1 Review

17 Publications
WT1 Antibody (6F-H2) - BSA Free
NB110-60011
Western Blot: WT1 Antibody (6F-H2) [NB110-60011] - Hypomethylation of the Intron 1 CpG island results in WT1 expression. RNA was isolated from K562 and U937 cells growing under atmospheric conditions (Normox) and from U937 cells growing in 1% O2 (Hypox) and was analyzed by RT-PCR using primers that span exon 5 of the WT1 mRNA. Ribosomal RNA 36B4 is used as a positive control, and molecular weight markers (MW) are shown. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119837), licensed under a CC-BY license.Immunohistochemistry-Paraffin: WT1 Antibody (6F-H2) [NB110-60011] - Staining of human kidney glomerulus tissue. Heat mediated antigen retrieval was performed by heating in citrate buffer (pH 6) at 95C for 20 minutes. Image from verified customer review.

Mouse Monoclonal
Species Human
Applications WB, Simple Western, ICC/IF

     3 Reviews

15 Publications
Alpha Actinin 4 Antibody (4D10)
H00000081-M01
Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on HeLa cell. Antibody concentration 20 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

4 Publications
Serpin A8/Angiotensinogen Antibody - BSA Free
NBP1-30027
Western Blot: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - Analysis of Angiotensinogen in human kidney lysate.Immunocytochemistry/Immunofluorescence: Serpin A8/Angiotensinogen Antibody [NBP1-30027] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Angiotensinogen at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

11 Publications
MLYCD Antibody
NBP1-32797
Immunohistochemistry-Paraffin: MLYCD Antibody [NBP1-32797] - Mouse muscle. MCD antibody [N2C1], Internal dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Western Blot: MLYCD Antibody [NBP1-32797] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MCD antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     1 Review

TRPC6 Antibody - BSA Free
NBP1-77260
Western Blot: TRPC6 Antibody [NBP1-77260] - Mouse lung tissue lysate with TRPC6 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.Immunocytochemistry/Immunofluorescence: TRPC6 Antibody [NBP1-77260] - Immunofluorescence of TRPC6 in Mouse Lung cells with TRPC6 antibody at 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
Nephrin Antibody [Unconjugated]
AF3159
Nephrin was detected in perfusion fixed frozen sections of mouse kidney using 10 µg/mL Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <A class=Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Nephrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Mouse
Applications IHC

57 Publications
ACE/CD143 Antibody [Unconjugated]
AF1513
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for <A class=

Goat Polyclonal
Species Mouse
Applications WB, Simple Western, Flow

     1 Review

7 Publications
Insulin Antibody (182410) [Unconjugated]
MAB1417
Insulin was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

24 Publications
Nucleostemin Antibody [Unconjugated]
AF1638
Western blot shows lysates of PC-3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Nucleostemin was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC

9 Publications
Complement Component C3d Antibody [Unconjugated]
AF2655
Complement Component C3d was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href= Complement Component C3d was detected in perfusion fixed paraffin-embedded sections of rat kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Goat Polyclonal
Species Mouse, Rat
Applications WB, IHC

57 Publications
Albumin Antibody (188835) [Unconjugated] - Serum
MAB1455
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Simple Western, IHC

     3 Reviews

54 Publications
Synaptopodin Antibody - BSA Free
NBP2-39100
Western Blot: Synaptopodin Antibody [NBP2-39100] - Representative western blotting results of nephrin and synaptopodin expression level in cultured podocytes as indicates. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41419-020-2481-5), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Synaptopodin Antibody [NBP2-39100] - Mouse brain tissue with SYNPO antibody at 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

10 Publications
TNF-alpha [Unconjugated]
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

810 Publications
Glycophorin A Antibody (R10) [Unconjugated]
MAB1228
TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228, filled histogram) or isotype control antibody (Catalog # <a class=Glycophorin A was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications Flow, IHC, IP

5 Publications
RAPGEF5 Antibody
NBP2-57362
Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF

Podocin/NPHS2 Antibody (JB51-33)
NBP2-75624
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of paraffin-embedded mouse kidney tissue using anti-Podocin/NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Flow Cytometry: Podocin/NPHS2 Antibody (JB51-33) [NBP2-75624] - Analysis of 293T cells with NPHS2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

6 Publications
non-muscle Myosin IIA Antibody (2B3)
H00004627-M03
Western Blot: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3. Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa). Lane 2: Non-transfected lysate.Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (2B3) [H00004627-M03] - Bovine ovarian granulosa cells. Anti Myosin IIA immunofluorescent staining (green). DNA in counterstained with DAPI (blue). Control was obtained by omitting the primary antibody. Fixation: 4% PF for 7 minutes at rt. Permeabilization: 0.1% Triton-X in PBS for 7 minutes. Blocking: 20% donkey serum, 1% BSA in PBS, 1 hour at rt. Primary antibody: 1:50 or 1:100 in 1% BSA/PBS over night at 4C. Secondary antibody: donkey anti mouse -Alexa 488 (Invitrogen), 1:500. DNA Counterstaining: mounting medium with DAPI. Negative CTRL was performed by omitting the primary antibody, images were captured under an epifluorescence microscope. Image from verified customer review.

Mouse Monoclonal
Species Human, Bovine
Applications WB, ELISA, ICC/IF

     1 Review

2 Publications