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Migraine Disorders: Disease Bioinformatics

Research of Migraine Disorders has been linked to Headache, Pain, Migraine With Aura, Tension Headache, Depressive Disorder. The study of Migraine Disorders has been mentioned in research publications which can be found using our bioinformatics tool below. Researched pathways related to Migraine Disorders include Pathogenesis, Sensitization, Vasoconstriction, Reflex, Vasodilation. These pathways complement our catalog of research reagents for the study of Migraine Disorders including antibodies and ELISA kits against 5-HT1B SEROTONIN RECEPTOR, ATP1A2, CACNA1A, CALCA, CAT.

Top Research Reagents

We have 3375 products for the study of Migraine Disorders that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB300-605
Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 ug/mL for 16 hours (right lane) and 10 uL of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.Immunohistochemistry-Paraffin: iNOS Antibody [NB300-605] - Immunohistochemistry was performed on normal deparaffinized human Lung tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, IB

     3 Reviews

130 Publications
NB100-56350
Western Blot: 5-HT1B Antibody [NB100-56350] - Analysis of 5-HT1B in human brain lysate with anti-5-HT1BR pcAb (NB100-56350). A protein band of approximate molecular weight of 40-41kDa was detected.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB

1 Publication
H00004854-M01
ELISA: Notch-3 Antibody (1G5) [H00004854-M01] - Detection limit for recombinant GST tagged NOTCH3 is approximately 0.03ng/ml as a capture antibody.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

     1 Review

13 Publications
NBP3-30941
Immunohistochemistry: CACNA1A Antibody [NBP3-30941] -  Immunohistochemical staining of normal human brain tissue using alpha1A calcium channel antibody (Cat. No. NBP3-30941) at 15 ug/ml

Rabbit Polyclonal
Species Human, Rat
Applications WB, IHC

NBP3-32061
Western Blot: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061] - Western blot analysis of Sodium Potassium ATPase Alpha 2 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 2 antibody (NBP3-32061) at 1/1,000 dilution.<br/><br/> Lane 1: Mouse heart tissue lysate<br/> Lane 2: Rat heart tissue lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 112 kDa<br/> Observed band size: 100 kDa<br/><br/> Exposure time: 24 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32061) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.Immunohistochemistry: Sodium Potassium ATPase Alpha 2 Antibody (JE34-00) [NBP3-32061] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-Sodium Potassium ATPase Alpha 2 antibody (NBP3-32061) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32061) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, IHC

NBP1-51843
Immunocytochemistry/Immunofluorescence: Aurora A Antibody [NBP1-51843] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti- (NBP1-51843) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution overnight at 4C and detected with an anti-mouse Dylight 550 (Red) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.Simple Western: Aurora A Antibody [NBP1-51843] - Simple Western lane view shows a specific band for Aurora A in 0.5 mg/ml of HeLa lysate.  This experiment was performed under reducing conditions using the 12-230kDa separation system.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

     1 Review

7 Publications
NBP1-76289
Western Blot: NLRP5 Antibody [NBP1-76289] - Mouse brain tissue lysate with NLRP5 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.Immunocytochemistry/Immunofluorescence: NLRP5 Antibody [NBP1-76289] - Immunofluorescence of NALP5 in human brain tissue with NALP5 antibody at 20 ug/mL.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NBP1-86616
Immunohistochemistry-Paraffin: CRAT Antibody [NBP1-86616] - Analysis in human testis and lymph node tissues.  Corresponding CRAT RNA-seq data are presented for the same tissues.Western Blot: CRAT Antibody [NBP1-86616] - Analysis using Anti-CRAT antibody NBP1-86616 (A) shows similar pattern to independent antibody NBP1-86615 (B).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

2 Publications
NB100-1533
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY license.Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

10 Publications
AF1052
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=EN‑RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, Flow

     3 Reviews

7 Publications
AF3398
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC

     3 Reviews

15 Publications
AF3847
Notch‑4 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human Notch‑4 Intracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3847) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, ICC

2 Publications
KGE007
N/A Substance P [Biotin]N/A Substance P [Biotin]


Species Multi-Species
Applications ELISA

37 Publications
210-TA
Recombinant Human TNF-alpha (Catalog # 210-TA) has a molecular weight (MW) of 53.1 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Recombinant Human TNF-alpha  (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 25‑100 pg/mL.


Species Human
Applications BA

     3 Reviews

811 Publications
7954-GM/CF
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 6-30 pg/mL.


Species Human
Applications BA

3 Publications
NBP2-42388
Immunohistochemistry-Paraffin: LAMC2 Antibody (CL2980) [NBP2-42388] - Staining in human fallopian tube and liver tissues. Corresponding LAMC2 RNA-seq data are presented for the same tissues.Western Blot: LAMC2 Antibody (CL2980) [NBP2-42388] - Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMC2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

4 Publications
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human skeletal muscle.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP2-75515
Immunocytochemistry/Immunofluorescence: GOLPH3 Antibody [NBP2-75515] - Staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: GOLPH3 Antibody [NBP2-75515] - Analysis of paraffin-embedded rat epididymis tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB120-14817

Mouse Monoclonal
Species Human, Rat
Applications WB, ICC/IF, IHC

     1 Review

11 Publications