Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation (ChIP) 0.5 uM
|
| Application Notes |
This AFM primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 101 base pairs. |
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles. |
| Buffer |
10 uM in TE buffer. |
| Preservative |
No Preservative |
Background
Primers are designed to amplify DNA in intron 1 of the human AFM gene locus. AFM is expressed in the liver and therefore silent in most cell types. AFM is associated with modified histones targeted to repressed genes.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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Blogs on AFM primer
![Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/AFM-primer-Chromatin-Immunoprecipitation-NBP1-71653-img0003.jpg "Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/AFM-primer-Chromatin-Immunoprecipitation-NBP1-71653-img0004.jpg "Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
