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![Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324] - Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.](http://images.novusbio.com/fullsize/Kir6-1-Antibody-S366-60-Western-Blot-NBP2-59324-img0006.jpg "Western Blot: Kir6.1 Antibody (S366-60) [NBP2-59324] - Western Blot analysis of Rat Brain showing detection of ~45 kDa Kir6.1 protein using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~45 kDa. Other Band(s): ~100 kDa.")
![Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir6.1 Monoclonal Antibody, Clone S366-60 (NBP2-59324). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody (NBP2-59324) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir6.1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kir6-1-Antibody-S366-60-Immunocytochemistry-Immunofluorescence-NBP2-59324-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Kir6.1 Antibody (S366-60) [NBP2-59324] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Kir6.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/Kir6-1-Antibody-S366-60-Immunocytochemistry-Immunofluorescence-NBP2-59324-img0002.jpg)
![Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Native-Western-Blot-NBL1-12174-img0003.jpg "Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.")
![Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12174-img0002.jpg)
![Immunohistochemistry-Paraffin: Kir6.1 Antibody [NBP1-87710] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87710-img0008.jpg "Immunohistochemistry-Paraffin: Kir6.1 Antibody [NBP1-87710] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: Kir6.1 Antibody [NBP1-87710] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87710-img0005.jpg)
![Immunohistochemistry-Paraffin: Kir6.1 Antibody [NBP1-87710] - Staining of human testis shows very weak cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87710-img0006.jpg)
![Immunohistochemistry-Paraffin: Kir6.1 Antibody [NBP1-87710] - Staining of human heart muscle shows weak to moderate cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Immunohistochemistry-Paraffin-NBP1-87710-img0007.jpg)
![Western Blot: Kir6.1 Antibody [NBP2-85158] - WB Suggested Anti-KCNJ8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Western-Blot-NBP2-85158-img0001.jpg "Western Blot: Kir6.1 Antibody [NBP2-85158] - WB Suggested Anti-KCNJ8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate")
![Western Blot: Kir6.1 Antibody [NBP3-03677] - Analysis of extracts of various cell lines, using Kir6.1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/Kir6.1-Antibody-Western-Blot-NBP3-03677-img0001.jpg "Western Blot: Kir6.1 Antibody [NBP3-03677] - Analysis of extracts of various cell lines, using Kir6.1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Western Blot: KIR3DP1 Overexpression Lysate (Adult Normal) [NBP2-08750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIR3DP1.](http://images.novusbio.com/fullsize/KIR3DP1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08750-img0001.jpg "Western Blot: KIR3DP1 Overexpression Lysate (Adult Normal) [NBP2-08750] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for KIR3DP1.")
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Adult Placenta. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0009.jpg "Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Adult Placenta. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry-Paraffin: Kir2.2 Antibody [NBP2-87693] - Rabbit Anti-KCNJ12 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult Skeletal muscle. Observed Staining: Cytoplasm in hepatocytes. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy3. Se](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Immunohistochemistry-Paraffin-NBP2-87693-img0001.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - WB Suggested Anti-KCNJ12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human brain](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0002.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Pancreas. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0003.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Brain. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0004.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0005.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0006.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0007.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0008.jpg)
![Western Blot: Kir2.2 Antibody [NBP2-87693] - Host: Rabbit. Target Name: KCNJ12. Sample Type: Human Fetal Stomach. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/Kir2.2-Antibody-Western-Blot-NBP2-87693-img0010.jpg)
![Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901] - analysis of Human Cell lysates showing detection of Kir2.2 Potassium Channel protein using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-2-Antibody-S124B-38-Western-Blot-NBP2-12901-img0006.jpg "Western Blot: Kir2.2 Antibody (S124B-38) [NBP2-12901] - analysis of Human Cell lysates showing detection of Kir2.2 Potassium Channel protein using Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody, Clone S124B-38 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-2-Antibody-S124B-38-Immunohistochemistry-NBP2-12901-img0008.jpg)
![Immunohistochemistry: Kir2.2 Antibody (S124B-38) [NBP2-12901] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.2 Potassium Channel Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kir2-2-Antibody-S124B-38-Immunohistochemistry-NBP2-12901-img0007.jpg)
![Western Blot: Kir2.2 Antibody [NBP3-35430] - Western blot analysis of various lysates using Kir2.2 Rabbit pAb at 1:300 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35430_rabbit-kir2-2-pab-9122024159554.jpg "Western Blot: Kir2.2 Antibody [NBP3-35430] - Western blot analysis of various lysates using Kir2.2 Rabbit pAb at 1:300 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.")