| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51641SS | |||
| Azide and BSA Free | NBP2-80949 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52563-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-02984-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74199 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7714-SP | |||
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-NBP1-51641-img0015.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.")
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Intracellular-NBP1-51641-img0014.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51641_mouse-monoclonal-sirtuin-1-sirt1-antibody-1f3-310202416165619.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP2-27205-img0003.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP2-27205-img0002.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-Western-Blot-NBP2-52563-img0004.jpg "Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.")
![Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Cochlear whole mount from wild-type and Sirt3-KO mice, immunolabeled with MYO7, SIRT3, and DAPI. The cross-hairs show the position from which the side view is taken. SIRT3 immunoreactivity only is shown in the right panels. IHC-WhMt image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-Immunohistochemistry-Whole-Mount-NBP2-52563-img0006.jpg)
![Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis of HeLa cells using SIRT3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-Flow-Cytometry-NBP2-52563-img0001.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against HEK293 (1) and SIRT3 (AA: 155-290)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-Western-Blot-NBP2-52563-img0003.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-Western-Blot-NBP2-52563-img0002.jpg)
![ELISA: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Black line: Control Antigen (100 ng); Purple line: Antigen(10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6B2A1-ELISA-NBP2-52563-img0005.jpg)
![Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Knockdown-Validated-NBP1-49540-img0007.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP1-49540-img0004.jpg)
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-NBP1-49540-img0005.jpg)
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP1-49540-img0006.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0007.jpg "Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Sirtuin 3/SIRT3 Rabbit pAb (NBP3-02984) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-02984-img0004.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Mouse kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0003.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of HepG2 cells, using Sirtuin 3/SIRT3 antibody (NBP3-02984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Sirtuin 3-SIRT3 Antibody-Western Blot-NBP3-02984-img0005.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of Mouse liver, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Human thyroid cancer using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0001.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Rat kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts from wild type(WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Knockout-Validated-NBP3-02984-img0008.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Western-Blot-H00023411-M01-img0014.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - SIRT1 monoclonal antibody (M01), clone 7B7 Analysis of SIRT1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Immunocytochemistry-Immunofluorescence-H00023411-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Analysis of monoclonal antibody to SIRT1 on formalin-fixed paraffin-embedded human lateral ventricle wall. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Immunohistochemistry-Paraffin-H00023411-M01-img0012.jpg)
![Sandwich ELISA: Sirtuin 1/SIRT1 Antibody (7B7) [H00023411-M01] - Detection limit for recombinant GST tagged SIRT1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-7B7-Sandwich-ELISA-H00023411-M01-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87038-img0004.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human lymph node shows moderate to strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0009.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Saining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0007.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody [NBP1-87038] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-Paraffin-NBP1-87038-img0008.jpg)
![Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Analysis in control (vector only transfected HEK293T lysate) and SIRT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415885).](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Western-Blot-NBP1-86013-img0008.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Analysis in control (vector only transfected HEK293T lysate) and SIRT5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415885).")
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human Stomach shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0011.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human heart muscle shows moderate to strong granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0005.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human pancreas shows weak cytoplasmic positivity in exocrine glandular.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0007.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human heart muscle shows moderate to strong granular cytoplasmic positivity.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0009.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0010.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody [NBP1-86013] - Analysis in human heart muscle and pancreas tissues using NBP1-86013 antibody. Corresponding SIRT5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Immunohistochemistry-Paraffin-NBP1-86013-img0012.jpg)
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-OTI2F1-Western-Blot-NBP2-01003-img0017.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-2F1-Immunocytochemistry-Immunofluorescence-NBP2-01003-img0013.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-2F1-Immunohistochemistry-Paraffin-NBP2-01003-img0014.jpg)
![Flow Cytometry: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-2F1-Flow-Cytometry-NBP2-01003-img0012.jpg)
![Western Blot: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT5.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0015.jpg)
![Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-2F1-Western-Blot-NBP2-01003-img0011.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP1-31029-img0011.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Analysis of methanol-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31029-img0008.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Cal27 xenograft, using SIRT3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP1-31029-img0009.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP1-31029-img0010.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Human endometrial carcinoma. SIRT3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP1-31029-img0007.jpg)
![Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Western-Blot-NB100-56372-img0004.jpg "Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372] - Immunoblots reveal the levels of sirtuins (SIRT4-7) in the OSCC cell lines HSC-3, UM-SCC-1, and UM-SCC-17B and in normal human oral keratinocytes (K). B-Actin served as loading control. Antibodies include SIRT4 (NB100-1406), SIRT5 (NB100-56372), and SIRT7 (NB100-56364). Image collected and cropped by CiteAb from the following publication (doi.org/10.1002/cncr.25676) licensed under a CC-BY license.")
![Western Blot: Sirtuin 5/SIRT5 Antibody [NB100-56372] - Analysis of Sirtuin 5/SIRT5 in A) human, B) mouse and C) rat intestine cell lysate using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Sirtuin-5-SIRT5-Antibody-Western-Blot-NB100-56372-img0003.jpg)