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![Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB100-56326-img0002.jpg "Western Blot: MeCP2 Antibody [NB100-56326] - Analysis of MeCP2 in HeLa cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB100-56326] - This antibody was tested in A431 cells at a 1:200 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56326-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0007.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Tissue section of mouse pancreas using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in majority of the cells from acini, ducts and Islets of Langerhans in the section.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB100-56326] - Mouse liver tissue using this antibody at 1:500 dilution. The antibody generated a very strong/specific nuclear staining with weak to negligible cytoplasmic signal in the hepatocytes, kupffer cells, and in the endothelial cells of blood vessels.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB100-56326-img0006.jpg)
![Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NB600-1101-img0006.jpg "Western Blot: MeCP2 Antibody [NB600-1101] - Analysis of HeLa cell extract.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1101-img0004.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NB600-1101] - Staining in the nucleus of rat brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NB600-1101-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NB600-1101] - Analysis of Methyl CpG Binding Protein 2 (green) showing staining in the nucleus of C6 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1101-img0003.jpg)
![Western Blot: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of MECP2 expression in Jurkat cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-1B11-Western-Blot-H00004204-M03-img0009.jpg "Western Blot: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of MECP2 expression in Jurkat cells.")
![Immunohistochemistry-Paraffin: MeCP2 Antibody (1B11) [H00004204-M03] - Human lymphoma tissue stained with MeCP2 Antibody (1B11). IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MeCP2-Antibody-1B11-Immunohistochemistry-Paraffin-H00004204-M03-img0010.jpg)
![Western Blot: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of MECP2 expression in HeLa S3 NE.](http://images.novusbio.com/fullsize/MeCP2-Antibody-1B11-Western-Blot-H00004204-M03-img0008.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody (1B11) [H00004204-M03] - Analysis of monoclonal antibody to MECP2 on FFPE human cerebral cortex. Antibody at 3 ug/mL.](http://images.novusbio.com/fullsize/MeCP2-Antibody-1B11-Immunohistochemistry-Paraffin-H00004204-M03-img0006.jpg)
![Sandwich ELISA: MeCP2 Antibody (1B11) [H00004204-M03] - Detection limit for recombinant GST tagged MECP2 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-1B11-Sandwich-ELISA-H00004204-M03-img0007.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0012.jpg "Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody (M01), clone 4B6.Lane 1: MECP2 transfected lysate(52.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: MeCP2 Antibody (4B6) [H00004204-M01] - Analysis of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Immunohistochemistry-Paraffin-H00004204-M01-img0007.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6 Analysis of MECP2 expression in MCF-7.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0009.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in NIH/3T3.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0010.jpg)
![Western Blot: MeCP2 Antibody (4B6) [H00004204-M01] - MECP2 monoclonal antibody (M01), clone 4B6. Analysis of MECP2 expression in rat muscle.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Western-Blot-H00004204-M01-img0011.jpg)
![Sandwich ELISA: MeCP2 Antibody (4B6) [H00004204-M01] - Detection limit for recombinant GST tagged MECP2 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/MeCP2-Antibody-4B6-Sandwich-ELISA-H00004204-M01-img0008.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0005.jpg "Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of tissue and cell lysates using rabbit pAb to MeCP2, NBP2-50060, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP2-50060] - Mouse brain section, which was cut at 45um on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with rabbit polyclonal antibody NBP2-50060. MeCP2 was mainly associated with methylated DNA in nucleus of neuron cells (green). Blue shows DAPI staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0003.jpg)
![Immunohistochemistry Free-Floating: MeCP2 Antibody [NBP2-50060] - Analysis of rat cerebellum section costained with rabbit pAb to MeCP2, NBP2-50060, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50060-img0004.jpg)
![Western Blot: MeCP2 Antibody [NBP2-50060] - Analysis of MeCP2 expression in nuclear extracts from mouse brain with NBP2-50060. This polyclonal antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2 of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP2-50060-img0002.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0014.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81027 (A) shows similar protein distribution across tissues to independent antibody NBP1-81028 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP1-81027] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81027-img0005.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0017.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0012.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0015.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81027] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81027-img0016.jpg)
![Western Blot: MeCP2 Antibody [NBP3-05542] - Western blot analysis of tissue and cell lysates using MeCP2 Antibody at a dilution of 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein. The MeCP2 proteins of rat and human origin are known to migrate slightly differently on SDS-PAGE gels compared to that of mouse, and as a result bands appear at somewhat different apparent molecular weights.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP3-05542-img0002.jpg "Western Blot: MeCP2 Antibody [NBP3-05542] - Western blot analysis of tissue and cell lysates using MeCP2 Antibody at a dilution of 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein. The MeCP2 proteins of rat and human origin are known to migrate slightly differently on SDS-PAGE gels compared to that of mouse, and as a result bands appear at somewhat different apparent molecular weights.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05542] - Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2 at a dilution of 1:2,000 in red and costained with mouse mAb to alpha-synuclein at a dilution of 1:1,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels the nuclei of neuronal cells while the alpha-synuclein antibody reveals alpha-synuclein protein concentrated in presynaptic regions.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05542-img0001.jpg)
![Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Western-Blot-NBP2-60787-img0001.jpg "Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (1:500, red), colabeled with anti-GFAP (1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Rat hippocampal section showing specific nuclear MeCP2immunolabeling in red and FOX3 in green.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (1:1000, red) in nuclei of neurons and specific labeling of GFAP (1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0003.jpg)
![Western Blot: MeCP2 Antibody [NBP3-05541] - Western blot analysis of tissue lysates using MeCP2 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate. Strong band at about 75kDa in rat and slightly lower in mouse nuclear enriched fractions corresponds to the MeCP2 protein. The same blot was simultaneously probed with chicken pAB to GAP-43 which detects GAP-43 protein in all preparations with apparent molecular weight of 43kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Western-Blot-NBP3-05541-img0002.jpg "Western Blot: MeCP2 Antibody [NBP3-05541] - Western blot analysis of tissue lysates using MeCP2 Antibody at a dilution of 1:1,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate. Strong band at about 75kDa in rat and slightly lower in mouse nuclear enriched fractions corresponds to the MeCP2 protein. The same blot was simultaneously probed with chicken pAB to GAP-43 which detects GAP-43 protein in all preparations with apparent molecular weight of 43kDa.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05541] - Immunofluorescent analysis of rat cerebellum section stained with MeCP2 Antibody at a dilution of 1:500 in red and costained with chicken pAb to calbindin at a dilution of 1:2,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels nuclei of neuronal cells to a variable degree, while calbindin is expressed in the dendrites and perikarya of Purkinje cells in the molecular layer of cerebellum.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05541-img0001.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81028 (A) shows similar protein distribution across tissues to independent antibody NBP1-81027 (B).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81028 (A) shows similar protein distribution across tissues to independent antibody NBP1-81027 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0015.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0010.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0013.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0014.jpg)
![Western Blot: MeCP2 [p Ser80] Antibody [NBP3-23406] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23406_rabbit-mecp2-p-ser80-pab-19920231252361.jpg "Western Blot: MeCP2 [p Ser80] Antibody [NBP3-23406] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: MeCP2 [p Ser80] Antibody [NBP3-23406] - Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-80) rabbit polyclonal antibody (NBP3-23406) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23406_rabbit-mecp2-p-ser80-pab-19920231915413.jpg)
![Western Blot: MeCP2 Antibody [NBP3-23405] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23405_rabbit-mecp2-pab-19920231410122.jpg "Western Blot: MeCP2 Antibody [NBP3-23405] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-23405] - Immunocytochemical labeling of MeCP2 in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (C-terminus) rabbit polyclonal antibody (NBP3-23405) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23405_rabbit-mecp2-pab-1192023166533.jpg)