| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NB200-112F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-77452SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94822-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94900-0.02ml | |||
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0035.jpg "Western Blot: TSG101 Antibody (4A10) [NB200-112] - Whole cell lysates (WCL) or exosome sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. TSG101 Antibody (NB200-112) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.")
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Immunohistochemistry-Paraffin-NB200-112-img0026.jpg)
![Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] (NB200-112) detects TSG101 protein by flow cytometry analysis.Sample: THP-1 cell.Black: Unlabelled sample was used as a control.Red: TSG101 antibody [4A10] (NB200-112).Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Flow-Cytometry-NB200-112-img0027.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - A. 30 ug NIH-3T3 whole cell lysate/extract. B. 30 ug JC whole cell lysate/extract. C. 30 ug BCL-1 whole cell lysate/extract.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0009.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0018.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0020.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0033.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. Western blot analysis of exosomes revealed differential expression of TSG101 between HCT116-R exosomes and HCT116-P exosomes. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-53063-y), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Western-Blot-NB200-112-img0034.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - Human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (NB200-112).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Immunohistochemistry-Paraffin-NB200-112-img0024.jpg)
![ELISA: TSG101 Antibody (4A10) [NB200-112] - ELISA detection of TSG101 for capture at a concentration of 5 ug/mL and TSG101 for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-ELISA-NB200-112-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TSG101 Antibody (4A10) [NB200-112] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] diluted at 1:500.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-Knockdown-Validated-NB200-112-img0014.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of pECs-EVs from different women in secretory & proliferative phase of cycle. Representative TEM images of pECs-EVs from secretory (A,B) & proliferative phase (C,D). Scale bars: 50 nm (A,C); 100 nm (B,D). Representative nanoparticle tracking analysis plots of pECs-EVs from secretory (E) & proliferative phase (F). Western blot showing the presence of different canonical EV markers (CD63, CD9, Alix & TSG101) in secretory & proliferative phase pECs-EVs (n = 4) (G). Negative EV markers used were Calnexin (ER), beta -tubulin & the cytosolic form of DCXR (H). The same markers were evaluated in pECs protein extract as controls for EV enrichment of these markers. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32483153), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-31020241529330.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Characterization of exosomes. (A) Representative TEM images of exosomes at 22000x magnification (Left) HCT116-R exosomes & (Right) HCT116-P exosomes. (B) Enlarged planar view of a cup-shaped structure for single exosome in our samples at 87000x magnification. (C) Western blot analysis of exosomes revealed differential expression of TSG101, CD9, & CD63 between HCT116-R exosomes & HCT116-P exosomes. (D) Average size distribution & zeta potential of exosomes measured in Zetasizer. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31712601), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-310202415392526.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - EPS15 depletion affects late endosomal maturation.(A) Co-depletion of SPOPL an EPS15 has an additive effect on influenza A virus infection. (B) EPS15 depletion stabilizes ESCRT components HRS, STAM & TSG101. (C) EPS15 depletion affects LDL uptake in cells resulting in an accumulation of LDL in enlarged vacuoles (upper panel). Late endosomes, visualized by life-cell microscopy of GFP-RAB7, are enlarged in cells depleted of EPS15 (lower panel).DOI://dx.doi.org/10.7554/eLife.13841.016 Image collected & cropped by CiteAb from the following publication (//elifesciences.org/articles/13841), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-310202415394543.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (4A10) [NB200-112] - TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.TSG101 stained by TSG101 antibody [4A10] (NB200-112) diluted at 1:50.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-910202420131256.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-910202420104410.jpg)
![Western Blot: TSG101 Antibody (4A10) [NB200-112] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (NB200-112) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nb200-112_mouse-monoclonal-tsg101-antibody-4a10-91020242041227.jpg)
![Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-77452-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-77452] - Total protein from human HeLa, HepG2, mouse 3T3 and rat PC12 cells lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-TSG101 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-77452] - Analysis of a FFPE tissue section of the human small intestine using 1:200 dilution of TSG101 antibody (NBP2-77452). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated staining mainly in the cytoplasm and membrane of glandular cells.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-77452-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-77452] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-TSG101 at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-77452-img0003.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0007.jpg "Western Blot: Endothelial Lipase Antibody [NB400-111] - Human endothelial lipase detected in transfected 293 lysates (MW 57 kDa)")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody [NB400-111] - Antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Immunocytochemistry-Immunofluorescence-NB400-111-img0001.jpg)
![Western Blot: Endothelial Lipase Antibody [NB400-111] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY LIPG cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-Western-Blot-NB400-111-img0006.jpg)
![Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Western-Blot-NBP2-67884-img0010.jpg "Western Blot: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysateLane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0009.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0007.jpg)
![Flow Cytometry: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Flow-Cytometry-NBP2-67884-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody (JJ0900) [NBP2-67884] - Staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunocytochemistry-Immunofluorescence-NBP2-67884-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Immunohistochemistry-Paraffin-NBP2-67884-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: TSG101 Antibody (JJ0900) [NBP2-67884] - Analysis of TSG101 with anti-TSG101 antibody [JJ0900] at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2/3: TSG101 knockdown Hela whole cell lysate (10 ug). NBP2-67884 was shown to specifically react with TSG101 in wild-type Hela cells. Weakened bands were observed when TSG101 knockdown samples were tested. Wild-type and TSG101 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Knockdown-Validated-NBP2-67884-img0012.jpg)
![Simple Western: TSG101 Antibody (JJ0900) [NBP2-67884] - Simple Western Lane view shows K562 whole cell lysate (WCL). A specific band was detected for TSG101 antibody (NBP2-67884) at approximately 53 kDa (as indicated) using 1:50 ug/mL of TSG101 antibody. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/TSG101-Antibody-JJ0900-Simple-Western-NBP2-67884-img0013.jpg)
![Western Blot: TSG101 Antibody [NBP2-94822] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 40s.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-94822-img0002.jpg "Western Blot: TSG101 Antibody [NBP2-94822] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 40s.")
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Rat kidney using TSG101/VPS23 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0007.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Human colon carcinoma using TSG101/VPS23 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0005.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Mouse kidney using TSG101/VPS23 Rabbit pAb t dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0004.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94822] - Rat kidney using TSG101/VPS23 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94822-img0006.jpg)
![Immunoprecipitation: TSG101 Antibody [NBP2-94822] - Analysis of 200ug extracts of HeLa cells using 3ug TSG101/VPS23 antibody. Western blot was performed from the immunoprecipitate using TSG101/VPS23 antibody (A1692) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunoprecipitation-NBP2-94822-img0008.jpg)
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0005.jpg "Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/VPS41-Antibody-Immunohistochemistry-Paraffin-NB100-2425-img0004.jpg)
![Western Blot: TSG101 Antibody [NBP2-94900] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.](http://images.novusbio.com/fullsize/TSG101-Antibody-Western-Blot-NBP2-94900-img0004.jpg "Western Blot: TSG101 Antibody [NBP2-94900] - Analysis of extracts of various cell lines, using TSG101 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: TSG101 Antibody [NBP2-94900] - Analysis of L929 cells using TSG101 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-94900-img0001.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94900] - Analysis of 150ug extracts of HeLa cells using TSG101 at a dilition of 1:1000.](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94900-img0003.jpg)
![Immunohistochemistry-Paraffin: TSG101 Antibody [NBP2-94900] - Paraffin-embedded Mouse kidney using TSG101 .](http://images.novusbio.com/fullsize/TSG101-Antibody-Immunohistochemistry-Paraffin-NBP2-94900-img0002.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TSG101-Antibody-4A10-RB-Western-Blot-NBP3-13656-img0001.jpg "Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-9102024202190.jpg)
![Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] (NBP3-13656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-91020242001026.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TSG101 Antibody (4A10-RB) [NBP3-13656] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10-RB] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13656_rabbit-tsg101-mab-4a10-rb-1610202419414543.jpg)
![Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Western-Blot-NBP3-16322-img0002.jpg "Western Blot: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of extracts of various cell lines, using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Mouse testis using Integrin alpha 4/CD49d (ITGA4/CD49d) Rabbit mAb (NBP3-16322) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Immunohistochemistry-Paraffin-NBP3-16322-img0001.jpg)
![Immunoprecipitation: Integrin alpha 4/CD49d Antibody (8M8C2) [NBP3-16322] - Analysis of 300ug extracts of Jurkat cells using 3ug Integrin alpha 4/CD49d antibody (NBP3-16322). Western blot was performed from the immunoprecipitate using Integrin alpha 4/CD49d antibody (NBP3-16322) at a dilition of 1:500.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-8M8C2-Immunoprecipitation-NBP3-16322-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0004.jpg "Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human endometrium shows moderate granular cytoplasmic positivity in in glandular cells.")
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0002.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human urinary bladder shows moderate granular cytoplasmic positivity in in urothelial cells.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS4B Antibody [NBP2-49263] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Immunohistochemistry-Paraffin-NBP2-49263-img0005.jpg)
![Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-9F10-Flow-Cytometry-NBP1-43398-img0001.jpg "Flow Cytometry: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Staining of normal human peripheral blood cells with 0.125 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.125 ug of Anti-Human CD49d Purified (filled histogram) followed by Anti-Mouse IgG FITC. Cells in the lymphocyte gate were used for analysis.")
![Immunofluorescence: Integrin alpha 4/CD49d Antibody (9F10) [NBP1-43398] - Analysis was performed using 70% confluent log phase MOLT-4 cells.](http://images.novusbio.com/fullsize/Integrin-alpha-4-CD49d-Antibody-9F10-Immunofluorescence-NBP1-43398-img0002.jpg)
![Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.](http://images.novusbio.com/fullsize/VPS4B-Antibody-Western-Blot-NBP1-19119-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: VPS4B Antibody [NBP1-19119] - Whole cell lysate from HeLa and 293T cells. VPS4B was also immunoprecipitated by rabbit anti-VPS4B antibody NBP1-19118.")