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![Knockdown Validated: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Knockdown-Validated-NB500-206-img0004.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Western-Blot-NB500-206-img0002.jpg)
![Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Simple-Western-NB500-206-img0003.jpg)
![Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0008.jpg "Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.")
![<b>Biological Strategies Validation. </b>Western Blot: Caspase-7 Antibody (25B881.1) [NB100-56529] - Analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Western-Blot-NB100-56529-img0009.jpg)
![Immunohistochemistry-Paraffin: Caspase-7 Antibody (25B881.1) [NB100-56529] - Human tonsil stained with Caspase-7 antibody at 5 ug/ml. Novus' human tonsil tissue slide was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-25B881-1-Immunohistochemistry-Paraffin-NB100-56529-img0007.jpg)
![Western Blot: Caspase-7 Antibody (7-1-11) [NBP1-19229] - Analysis of Caspase-7, mAb (7-1-11): Lane1: MW Marker; Lane 2: Jurkat ; Lane 3: Jurkat plus Staurosporine; Lane 4: HeLa ; Lane 5: HeLa plus Nocodazole; Lane 6: 3T3 ; Lane 7: PC-12](http://images.novusbio.com/fullsize/Caspase-7-Antibody-7-1-11-Western-Blot-NBP1-19229-img0008.jpg "Western Blot: Caspase-7 Antibody (7-1-11) [NBP1-19229] - Analysis of Caspase-7, mAb (7-1-11): Lane1: MW Marker; Lane 2: Jurkat ; Lane 3: Jurkat plus Staurosporine; Lane 4: HeLa ; Lane 5: HeLa plus Nocodazole; Lane 6: 3T3 ; Lane 7: PC-12")
![Immunohistochemistry: Caspase-7 Antibody (7-1-11) [NBP1-19229] - Human spleen tissue stained with Caspase-7, mAb (7-1-11) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-7-1-11-Immunohistochemistry-NBP1-19229-img0007.jpg)
![Immunohistochemistry: Caspase-7 Antibody (7-1-11) [NBP1-19229] - Human colon tissue stained with Caspase-7, mAb (7-1-11) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-7-1-11-Immunohistochemistry-NBP1-19229-img0005.jpg)
![Immunohistochemistry: Caspase-7 Antibody (7-1-11) [NBP1-19229] - Human placenta tissue stained with Caspase-7, mAb (7-1-11) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-7-1-11-Immunohistochemistry-NBP1-19229-img0006.jpg)
![Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Analysis of human Jurkat and Jurkat induced cell lysates probed with AAM-137](http://images.novusbio.com/fullsize/Caspase-7-Antibody-10-1-62-Western-Blot-NBP1-19230-img0004.jpg "Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Analysis of human Jurkat and Jurkat induced cell lysates probed with AAM-137")
![Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human spleen tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-10-1-62-Immunohistochemistry-NBP1-19230-img0002.jpg)
![Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human colon tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-10-1-62-Immunohistochemistry-NBP1-19230-img0001.jpg)
![Western Blot: Caspase-7 Antibody (S03-0F2) [NBP3-14866] - Western blot detection of Caspase-7 in A549, MCF-7, 293T cell lysates using Caspase-7 Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-S03-0F2-Western-Blot-NBP3-14866-img0001.jpg "Western Blot: Caspase-7 Antibody (S03-0F2) [NBP3-14866] - Western blot detection of Caspase-7 in A549, MCF-7, 293T cell lysates using Caspase-7 Rabbit mAb (1:1000 diluted). Predicted band size: 34kDa. Observed band size: 34kDa.")
![Immunohistochemistry-Paraffin: Caspase-7 Antibody (S03-0F2) [NBP3-14866] - Immunohistochemistry of Caspase-7 in paraffin-embedded Human Cholangiocarcinoma using Caspase-7 Rabbit mAb at dilution 1/50](http://images.novusbio.com/fullsize/Caspase-7-Antibody-S03-0F2-Immunohistochemistry-Paraffin-NBP3-14866-img0002.jpg)
![Western Blot: Caspase-7 Antibody (SR1559) [NBP3-21682] - Western blot analysis using NBP3-21682 in NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21682_rabbit-caspase-7-mab-sr1559-57202317215128.jpg "Western Blot: Caspase-7 Antibody (SR1559) [NBP3-21682] - Western blot analysis using NBP3-21682 in NIH/3T3 cell lysate.")
![ELISA: Rat Caspase-7 ELISA Kit (Colorimetric) [NBP2-75039] - Samples were spiked with high concentrations of Rat Caspase-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75039_rat-caspase-7-elisa-kit-colorimetric-132202416251718.jpg "ELISA: Rat Caspase-7 ELISA Kit (Colorimetric) [NBP2-75039] - Samples were spiked with high concentrations of Rat Caspase-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Caspase-7 ELISA Kit (Colorimetric) [NBP2-75039] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75039_rat-caspase-7-elisa-kit-colorimetric-432024983577.jpg)
![ELISA: Human Caspase-7 ELISA Kit (Colorimetric) [NBP2-75037] - Samples were spiked with high concentrations of Human Caspase-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75037_human-caspase-7-elisa-kit-colorimetric-132202416133661.jpg "ELISA: Human Caspase-7 ELISA Kit (Colorimetric) [NBP2-75037] - Samples were spiked with high concentrations of Human Caspase-7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Caspase-7 ELISA Kit (Colorimetric) [NBP2-75037] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75037_human-caspase-7-elisa-kit-colorimetric-432024963990.jpg)
![Western Blot: Caspase-7 Antibody (MCH3-5) [NBP3-30814] - Western blot analysis using caspase-7antibody on MCF-7 cells treated with thapsigarginfor 48 hours which are negative (-) and positive(+) for caspase-3.](http://images.novusbio.com/fullsize/nbp3-30814_mouse-caspase-7-mab-mch3-5-811202420592129.jpg "Western Blot: Caspase-7 Antibody (MCH3-5) [NBP3-30814] - Western blot analysis using caspase-7antibody on MCF-7 cells treated with thapsigarginfor 48 hours which are negative (-) and positive(+) for caspase-3.")