![Western Blot: ZNF143 Antibody (2B4) [H00007702-M01] - ZNF143 monoclonal antibody (M01), clone 2B4 Analysis of ZNF143 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ZNF143-Antibody-2B4-Western-Blot-H00007702-M01-img0010.jpg "Western Blot: ZNF143 Antibody (2B4) [H00007702-M01] - ZNF143 monoclonal antibody (M01), clone 2B4 Analysis of ZNF143 expression in Hela S3 NE.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, ChIP |
| Clone | 2B4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | ZNF143 (AAH20219, 1 a.a. ~ 626 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTEFPGGGMEAQHVTLCLTEAVTVADGDNLENMEGVSLQAVTLADGSTAYIQHNSKDAKLIDGQVIQLEDGSAAYVQHVPIPKSTGDSLRLEDGQAVQLEDGTTAFIHHTSKDSYDQSALQAVQLEDGTTAYIHHAVQVPQSDTILAIQADGTVAGLHTGDATIDPDTISALEQYAAKVSIDGSESVAGTGMIGENEQEKKMQIVLQGHATRVTAKSQQSGEKAFRCEYDGCGKLYTTAHHLKVHERSHTGDRPYQCEHAGCGKAFATGYGLKSHVRTHTGEKPYRCSEDNCTKSFKTSGDLQKHIRTHTGERPFKCPFEGCGRSFTTSNIRKVHVRTHTGERPYYCTEPGCGRAFASATNYKNHVRIHTGEKPYVCTVPGCDKRFTEYSSLYKHHVVHTHSKPYNCNHCGKTYKQISTLVMHKRTAHNDTEPIEEEQEAFFEPPPGQGEDVLKGSQITYVTGVEGDDVVSTQVATVTQSGLSQQVTLISQDGTQHVNISQADMQAIGNTITMVTQDGTPITVPAHDAVISSAGTHSVAMVTAEGTEGQQVAIVAQDLAAFHTASSEMGHQQHSHHLVTTETRPLTLVATSNGTQIAVQLGEQPSLEEAIRIASRIQQGETPGLDD |
| Specificity | ZNF143 - zinc finger protein 143 (clone pHZ-1) |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZNF143 |
| Purity | Ascites |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody reactivity against cell lysate and recombinant protein for WB. IT has been used for IF, IHC-P, and ELISA. ChIP usage reported in scientific literature (PMID: 25645053). |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | Ascites |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ZNF143-Antibody-2B4-Immunocytochemistry-Immunofluorescence-H00007702-M01-img0006.jpg "Immunocytochemistry/Immunofluorescence: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on HeLa cell. Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/ZNF143-Antibody-2B4-Immunohistochemistry-Paraffin-H00007702-M01-img0008.jpg "Immunohistochemistry-Paraffin: ZNF143 Antibody (2B4) [H00007702-M01] - Analysis of monoclonal antibody to ZNF143 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue. Antibody concentration 3 ug/ml.")
![ELISA: ZNF143 Antibody (2B4) [H00007702-M01] - Detection limit for recombinant GST tagged ZNF143 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ZNF143-Antibody-2B4-ELISA-H00007702-M01-img0011.jpg "ELISA: ZNF143 Antibody (2B4) [H00007702-M01] - Detection limit for recombinant GST tagged ZNF143 is approximately 0.1ng/ml as a capture antibody.")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")