![Western Blot: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Western-Blot-NBP2-81015-img0001.jpg "Western Blot: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 expression was assayed in lung adenocarcinoma cells after transfectionwith miR-33b or miR-NC, anti-miR-33b or anti-miR-NC in H1299 and A549 cells. Image submitted by verified customer review. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 2A8A6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Partial recombinant human ZEB1 (between residues 800-1124) expressed in E. coli [Uniprot P37275] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ZEB1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This antibody has had weak results in Western blot. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
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![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
| Publication using NBP2-81015 | Applications | Species |
|---|---|---|
| Radnaa E, Richardson L, Goldman B Et al. Stress signaler p38 mitogen-activated kinase activation: a cause for concern? Clin Sci (Lond) 2022-10-17 [PMID: 36250628] (CyTOF-ready, Human) Details: Citation using the Azide and BSA Free version of this antibody. |
CyTOF-ready | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
CyTOF | Human | 01/04/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for ZEB1 Antibody (NBP2-81015)Find related products by research area.
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Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1 By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can... Read full blog post. |
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Epithelial-Mesenchymal Transition (EMT) Markers Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a... Read full blog post. |
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Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel... Read full blog post. |
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CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post. |
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Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell surface cadherin glycoproteins. It is one member of the catenin family that includes alpha Catenin, beta Catenin, and gamma Catenin. Colocalization studies usi... Read full blog post. |
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| Gene Symbol | ZEB1 |
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-81015-img0002.jpg "Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - ZEB1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image from the standard format of this antibody.")
![Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81015-img0004.jpg "Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human recturm cancer tissues with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0008.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Flow cytometric analysis of Hela cells using ZEB1 mouse mAb (green) and negative control (red). Image from the standard format of this antibody.")
![Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-81015-img0003.jpg "Immunohistochemistry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - IHC analysis of paraffin-embedded human cervical cancer tissues with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0005.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on Jurkat with ZEB1 Antibody (2A8A6) NBP2-23484 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody (R&D Systems, F0102B). Image from the standard format of this antibody.")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0006.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on U-87 cells with ZEB1 [2A8A6] Antibody NBP2-23484AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 700.")
![Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-81015-img0007.jpg "Flow Cytometry: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - An intracellular stain was performed on with A549 cells with ZEB1 Antibody NBP2-23484PE and a a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.")
![CyTOF-ready: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Analysis of ZEB1 antibody on Human tissue. ZEB1 expression in the epithelial layers (2 outer sides) and mesenchymal cells (middle area). Image from verified customer review.](http://images.novusbio.com/fullsize/ZEB1-Antibody-2A8A6-Azide-and-BSA-Free-CyTOF-ready-NBP2-81015-img0009.jpg "CyTOF-ready: ZEB1 Antibody (2A8A6) - Azide and BSA Free [NBP2-81015] - Analysis of ZEB1 antibody on Human tissue. ZEB1 expression in the epithelial layers (2 outer sides) and mesenchymal cells (middle area). Image from verified customer review.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
