![Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - Anti-UBE2I/Ubc9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NBP3-00096-img0003.jpg "Western Blot: UBE2I/Ubc9 Antibody [NBP3-00096] - Anti-UBE2I/Ubc9 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lane C: NIH-3T3 Whole Cell Lysate Lane D: PC12 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human UBE2I/Ubc9 (Accession#: P63279; Met1-Ser158) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBE2I |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for UBE2I/Ubc9 Antibody (NBP3-00096)Find related products by research area.
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| Gene Symbol | UBE2I |
![Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human UBE2I/Ubc9 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00096-img0002.jpg "Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP3-00096] - Immunofluorescence staining of UBE2I/Ubc9 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human UBE2I/Ubc9 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.")
![Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I/Ubc9 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate Lane B: 0.5 mg Hela Whole Cell Lysate Lane C: 0.5 mg A431 Whole Cell Lysate 2 ul anti-UBE2I/Ubc9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UBE2I/Ubc9 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunoprecipitation-NBP3-00096-img0001.jpg "Immunoprecipitation: UBE2I/Ubc9 Antibody [NBP3-00096] - UBE2I/Ubc9 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate Lane B: 0.5 mg Hela Whole Cell Lysate Lane C: 0.5 mg A431 Whole Cell Lysate 2 ul anti-UBE2I/Ubc9 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-UBE2I/Ubc9 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 18 kDa")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")