![Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NBP1-86887-img0003.jpg "Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: IALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQ |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | UBE2I |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
||
| Control Peptide |
|
||
| Reviewed Applications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human and Mouse | 06/29/2017 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for UBE2I/Ubc9 Antibody (NBP1-86887)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Verified Customer 06/29/2017 |
||
| Application: | WB | |
| Species: | Human and Mouse |
| Gene Symbol | UBE2I |
![Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86887-img0006.jpg "Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0010.jpg "Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.")
![Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Western-Blot-NBP1-86887-img0004.jpg "Western Blot: UBE2I/Ubc9 Antibody [NBP1-86887] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0007.jpg "Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human tonsil shows moderate to strong nuclear positivity in non - germinal center cells.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0008.jpg "Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human tonsil shows moderate to strong nuclear positivity in non - germinal center cells.")
![Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/UBE2I-Ubc9-Antibody-Immunohistochemistry-Paraffin-NBP1-86887-img0009.jpg "Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.")
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
