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UbcH5b/UBE2D2 Antibody (4A1)

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Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1. Analysis of UBE2D2 expression in Raw 264.7.
Immunohistochemistry-Paraffin: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Analysis of monoclonal antibody to UBE2D2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1. Analysis of UBE2D2 expression in PC-12.
Western Blot: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - UBE2D2 monoclonal antibody (M02), clone 4A1 Analysis of UBE2D2 expression in NIH/3T3.
Sandwich ELISA: UbcH5b/UBE2D2 Antibody (4A1) [H00007322-M02] - Detection limit for recombinant GST tagged UBE2D2 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
4A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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UbcH5b/UBE2D2 Antibody (4A1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
UBE2D2 (NP_003330, 1 a.a. ~ 94 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWS
Specificity
UBE2D2 (4A1)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UBE2D2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using H00007322-M02.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for UbcH5b/UBE2D2 Antibody (4A1)

  • E2(17)KB2
  • EC 6.3.2.19
  • PUBC1
  • UBC4/5
  • UBC4ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5)
  • UBC5B
  • UBCH4
  • UbcH5b
  • UBE2D2
  • Ubiquitin carrier protein D2
  • ubiquitin-conjugating enzyme E2 D2
  • Ubiquitin-conjugating enzyme E2(17)KB 2
  • Ubiquitin-conjugating enzyme E2-17 kDa 2
  • ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UbcH5b/UBE2D2 Antibody (H00007322-M02)(2)

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Bioinformatics

Gene Symbol UBE2D2
Entrez
OMIM
Uniprot