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Tumstatin/COL4A3 Antibody - Azide and BSA Free

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Western Blot: Tumstatin/COL4A3 Antibody [NBP3-02967] - Western blot analysis of extracts of Mouse kidney, using Tumstatin/COL4A3 antibody (NBP3-02967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western Blot: Tumstatin/COL4A3 Antibody [NBP3-02967] - Western blot analysis of extracts of Mouse kidney, using Tumstatin/COL4A3 antibody (NBP3-02967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of PC-12 cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of NIH/3T3 cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of HeLa cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-02967-20ul
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Tumstatin/COL4A3 Antibody - Azide and BSA Free Summary

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1555-1669 of human Tumstatin/COL4A3 (NP_000082.2). AIAIAVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRASPFLECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMKKR
Specificity
Possible crossreactivity to whole COL4A3 molecule.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COL4A3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500 - 1:1000
Theoretical MW
162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for Tumstatin/COL4A3 Antibody - Azide and BSA Free

  • COL4A3
  • collagen alpha-3(IV) chain
  • collagen IV, alpha-3 polypeptide
  • collagen, type IV, alpha 3 (Goodpasture antigen)
  • Goodpasture antigen
  • Tumstatin

Background

COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL4A3