![Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-TAGLN-247-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47688-img0002.jpg "Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)")
| Reactivity | Hu, Mu, Po, Bv, RbSpecies Glossary |
| Applications | IHC |
| Clone | TAGLN/247 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44435). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant full-length human Transgelin/TAGLN/SM22 alpha protein. (Uniprot: Q01995) |
| Localization | Cytoplasmic |
| Specificity | This monoclonal antibody recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TAGLN |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TAGLN |
![Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)](http://images.novusbio.com/fullsize/Transgelin-TAGLN-SM22-alpha-Antibody-TAGLN-247-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47688-img0001.jpg "Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)")
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")