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TRAILR2/TNFRSF10B Antibody (OTI2A5)

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Western Blot: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Western Blot: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Carcinoma of Human thyroid tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAIL R2/TNFRSF10B Antibody (2A5) [NBP2-46148] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC
Clone
OTI2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74578AF350
Alexa Fluor 405
NBP2-74578AF405
Alexa Fluor 488
NBP2-74578AF488
Alexa Fluor 532
NBP2-74578AF532
Alexa Fluor 594
NBP2-74578AF594
Alexa Fluor 647
NBP2-74578AF647
Alexa Fluor 700
NBP2-74578AF700
Alexa Fluor 750
NBP2-74578AF750
Biotin
NBP2-74578B
CoraFluor 1
NBP2-74578CL1
DyLight 350
NBP2-74578UV
DyLight 405
NBP2-74578V
DyLight 488
NBP2-74578G
DyLight 550
NBP2-74578R
DyLight 594
NBP2-74578DL594
DyLight 650
NBP2-74578C
DyLight 680
NBP2-74578FR
DyLight 755
NBP2-74578IR
FITC
NBP2-74578F
HRP
NBP2-74578H
Janelia Fluor 525
NBP2-74578JF525
Janelia Fluor 549
NBP2-74578JF549
Janelia Fluor 646
NBP2-74578JF646
Janelia Fluor 669
NBP2-74578JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74578
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TRAILR2/TNFRSF10B Antibody (OTI2A5) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 203-411 of human TNFRSF10B(NP_671716) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF10B
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 500
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRAILR2/TNFRSF10B Antibody (OTI2A5)

  • CD262 antigen
  • CD262
  • death domain containing receptor for TRAIL/Apo-2L
  • Death receptor 5
  • DR5
  • DR5TRICK2B
  • Fas-like protein
  • KILLER/DR5
  • KILLERapoptosis inducing protein TRICK2A/2B
  • TNF-related apoptosis-inducing ligand receptor 2
  • TNFRSF10B
  • TRAIL R2
  • TRAIL receptor 2
  • TRAILR2
  • TRAIL-R2apoptosis inducing receptor TRAIL-R2
  • TRAILR2cytotoxic TRAIL receptor-2
  • TRICK2
  • TRICK2A
  • TRICKB
  • tumor necrosis factor receptor superfamily member 10B
  • tumor necrosis factor receptor superfamily, member 10b
  • tumor necrosis factor receptor-like protein ZTNFR9
  • ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TNFRSF10B