![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Formalin-fixed, paraffin-embedded human Placenta stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1782).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1782-Immunohistochemistry-Paraffin-NBP2-53273-img0002.jpg "Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Formalin-fixed, paraffin-embedded human Placenta stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1782).")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC, MA |
| Clone | THBD/1782 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54496) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant fragment (around aa 69-194) of human Thrombomodulin/BDCA-3 protein (exact sequence is proprietary) (Uniprot: P07204) |
| Localization | Cell Surface & Cytoplasmic |
| Marker | Endothelial Cell Marker |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | THBD |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA. Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 100 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Thrombomodulin/BDCA-3 Antibody (NBP2-53273)Find related products by research area.
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Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the... Read full blog post. |
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![Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1782).](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1782-Immunohistochemistry-Paraffin-NBP2-53273-img0001.jpg "Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Thrombomodulin/CD141 Monoclonal Antibody (THBD/1782).")
![Protein Array: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Analysis of Protein Array containing >19,000 full-length human proteins using Thrombomodulin/BDCA-3 Antibody (THBD/1782) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Thrombomodulin-BDCA-3-Antibody-THBD-1782-Protein-Array-NBP2-53273-img0004.jpg "Protein Array: Thrombomodulin/BDCA-3 Antibody (THBD/1782) [NBP2-53273] - Analysis of Protein Array containing >19,000 full-length human proteins using Thrombomodulin/BDCA-3 Antibody (THBD/1782) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
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