| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Clone | 31349 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta RIII Gly21-Asp781 Accession # AAA67061 |
| Specificity | Detects human TGF-beta RIII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant rat MIS RII, recombinant human (rh) TGF-beta RI, rhTGF-beta RII, recombinant mouse TGF-beta RII, or rhTGF-beta RIIb. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFBR3 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta . The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction. The Type III receptor binds TGF-beta 2 with the highest affinity. Other TGF-beta isoforms also bind the Type III receptor, but with lower affinities. Cellular responsiveness to TGF-beta 2 appears to be dependent on the presence of the Type III receptor which can interact with the signaling receptor complex. In addition to the transmembrane Type III receptor, a soluble form of the receptor is secreted by some cell types. The physiological role of this soluble receptor remains to be determined. The recombinant TGF-beta soluble receptor Type III binds the TGF-beta isoforms differentially in solution and exhibits TGF-beta antagonistic activities in vitro.
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| Publication using MAB242 | Applications | Species |
|---|---|---|
| Peng W, Maintz L, Allam J, Novak N Attenuated TGF-beta1 responsiveness of dendritic cells and their precursors in atopic dermatitis. Eur J Immunol, 2013-04-09;43(5):1374-82. 2013-04-09 [PMID: 23436698] (Flow Cytometry, Human) | Flow Cytometry | Human |
Secondary Antibodies |
Isotype Controls |
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TGF-beta RIII - a high affinity reservoir for TGF-beta I/II ligands with therapeutic potential TGF beta (transforming growth factor beta) is a superfamily of cytokines that participate in a variety of cellular processes including growth, proliferation, differentiation, and apoptosis. There are 3 classes of receptors for TGF beta cytokines an... Read full blog post. |
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TGF-beta RIII - A multi-functional regulator of the TGF-beta signaling pathway Transforming growth factor-beta receptor III (TGF-beta RIII) is one of three receptors for the secreted growth factor TGF-beta. Unlike type I and type II TGF-beta receptors, TGF-beta RIII does not participate directly in the propagation of intracel... Read full blog post. |
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