![Western Blot: TCF7L2 Antibody [NBP1-19083] - Western blot analysis of TCF7L2 (F) protein in D3H2LN TNBC cells after treatment with SCR or SID decoy peptide (2.5uM, 24 hours). SID decoy treatment inhibited full-length (~64 kDa) and, to a greater extent, short isoform variant (~53 kDa) TCF7L2 proteins in comparison to untreated or SCR treated controls. The band intensities for TCF7L2 in each lane were normalized to the GAPDH loading control and indicated in red. The result is representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11381) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TCF7L2-Antibody-Western-Blot-NBP1-19083-img0005.jpg "Western Blot: TCF7L2 Antibody [NBP1-19083] - Western blot analysis of TCF7L2 (F) protein in D3H2LN TNBC cells after treatment with SCR or SID decoy peptide (2.5uM, 24 hours). SID decoy treatment inhibited full-length (~64 kDa) and, to a greater extent, short isoform variant (~53 kDa) TCF7L2 proteins in comparison to untreated or SCR treated controls. The band intensities for TCF7L2 in each lane were normalized to the GAPDH loading control and indicated in red. The result is representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.11381) licensed under a CC-BY license.")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Synthetic peptide made to an internal portion of human TCF7L2 (within residues 150-200). [Swiss-Prot# Q9NQB0] |
| Predicted Species | Mouse (93%), Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TCF7L2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This TCF7L2 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~55 kDa. In ICC/IF cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-19083 | Applications | Species |
|---|---|---|
| Kwon YJ, Leibovitch BA, Bansal N et al. Targeted interference of SIN3A-TGIF1 function by SID decoy treatment inhibits Wnt signaling and invasion in triple negative breast cancer cells Oncotarget. 2017-10-24 [PMID: 29179446] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for TCF7L2 Antibody (NBP1-19083)Find related products by research area.
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| Gene Symbol | TCF7L2 |
![Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody [NBP1-19083] - TCF7L2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/TCF7L2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19083-img0004.jpg "Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody [NBP1-19083] - TCF7L2 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
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