![Western Blot: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Western Blot (1) HeLa and (2) HepG2 cell lysate using TCF4 Mouse Monoclonal Antibody (TCF4/1705).](http://images.novusbio.com/fullsize/TCF4-Antibody-TCF4-1705-Western-Blot-NBP3-07336-img0002.jpg "Western Blot: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Western Blot (1) HeLa and (2) HepG2 cell lysate using TCF4 Mouse Monoclonal Antibody (TCF4/1705).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IHC, MA |
| Clone | TCF4/1705 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08236) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary) (Uniprot: P15884 ) |
| Localization | Nucleus, Cytoplasm |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TCF4 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order antibody without BSA Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes |
| Theoretical MW | 60 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Formalin-fixed, paraffin-embedded human bladder stained with TCF4 Antibody (TCF4/1705) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/TCF4-Antibody-TCF4-1705-Immunohistochemistry-Paraffin-NBP3-07336-img0003.jpg "Immunohistochemistry-Paraffin: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Formalin-fixed, paraffin-embedded human bladder stained with TCF4 Antibody (TCF4/1705) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
![Protein Array: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TCF4 Mouse Monoclonal Antibody (TCF4/1705).](http://images.novusbio.com/fullsize/TCF4-Antibody-TCF4-1705-Protein-Array-NBP3-07336-img0001.jpg "Protein Array: TCF4 Antibody (TCF4/1705) [NBP3-07336] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TCF4 Mouse Monoclonal Antibody (TCF4/1705).")
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