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Syntaxin 1B Antibody (676204) [Unconjugated]

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Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CCD-1070Sk human foreskin fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Syntaxin 1B Monoclonal Antibody (Catalog # MAB6848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 1B at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CCD-1070Sk human foreskin fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
676204
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6848-SP
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Syntaxin 1B Antibody (676204) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Syntaxin 1B
Lys2-Lys264
Accession # P61266
Specificity
Detects human Syntaxin 1B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Syntaxin 1A and 1B1 is observed, and no cross-reactivity with rhSyntaxin 5, 6, 7, 8, 12, or 16 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STX1B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using MAB6848.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Syntaxin 1B Antibody (676204) [Unconjugated]

  • FP17469
  • STX1B
  • STX1B1
  • STX1B2
  • Syntaxin 1B
  • syntaxin 1B1
  • syntaxin 1B2
  • syntaxin-1B
  • syntaxin-1B1
  • syntaxin-1B2

Background

Syntaxin 1B/STX1B is an approximately 33 kDa member of the Syntaxin family.  It is a single-pass type IV transmembrane protein that is potentially involved in the docking of synaptic vesicles at presynaptic active zones. The cytoplasmic domain of Syntaxin 1B contains a coiled-coil Syntaxin domain (aa 29-180) which is implicated in the docking of synaptic vesicles with the presynaptic plasma membrane and a t-SNARE coiled coil domain (aa 191-253). Within the cytoplasmic domain, human Syntaxin 1B shares 100% aa sequence identity with mouse and rat Syntaxin 1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Syntaxin 1B
Antibody (676204) [Un ...
MAB6848
Syntaxin 1B Antibody (676204) [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB

Publications for Syntaxin 1B Antibody (MAB6848)(1)


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Bioinformatics

Gene Symbol STX1B
Uniprot