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Sirtuin 5/SIRT5 Antibody (OTI2F1)

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Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by SDS-PAGE and immunoblotted with anti-SIRT5 monoclonal antibody (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Genetic Strategies: Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Equivalent amounts of cell lysates (10ug per lane) of wild-type HeLa cells and SIRT5-Knockout HeLa cells were separated by ...read more
Immunocytochemistry/ Immunofluorescence: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT5.
Immunohistochemistry-Paraffin: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - Staining of paraffin-embedded Human Kidney tissue using anti-SIRT5 mouse monoclonal antibody.
Flow Cytometry: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SIRT5 antibody, and then ...read more
Western Blot: Sirtuin 5/SIRT5 Antibody (2F1) [NBP2-01003] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Western Blot: Sirtuin 5/SIRT5 Antibody (OTI2F1) [NBP2-01003] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT5 monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, KO
Clone
OTI2F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.24 mg/ml
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74199AF350
Alexa Fluor 405
NBP2-74199AF405
Alexa Fluor 488
NBP2-74199AF488
Alexa Fluor 532
NBP2-74199AF532
Alexa Fluor 594
NBP2-74199AF594
Alexa Fluor 647
NBP2-74199AF647
Alexa Fluor 700
NBP2-74199AF700
Alexa Fluor 750
NBP2-74199AF750
Allophycocyanin
NBP2-74199APC
Allophycocyanin/Cy7
NBP2-74199APCCY7
Biotin
NBP2-74199B
CoraFluor 1
NBP2-74199CL1
DyLight 350
NBP2-74199UV
DyLight 405
NBP2-74199V
DyLight 488
NBP2-74199G
DyLight 550
NBP2-74199R
DyLight 594
NBP2-74199DL594
DyLight 650
NBP2-74199C
DyLight 680
NBP2-74199FR
DyLight 755
NBP2-74199IR
FITC
NBP2-74199F
HRP
NBP2-74199H
Janelia Fluor 525
NBP2-74199JF525
Janelia Fluor 549
NBP2-74199JF549
Janelia Fluor 585
NBP2-74199JF585
Janelia Fluor 635
NBP2-74199JF635
Janelia Fluor 646
NBP2-74199JF646
Janelia Fluor 669
NBP2-74199JF669
PE
NBP2-74199PE
PE/Atto594
NBP2-74199PEATT594
PE/Cy5.5
NBP2-74199PECY55
PE/Cy7
NBP2-74199PECY7
PerCP
NBP2-74199PCP
mFluor Violet 450 SE
NBP2-74199MFV450
mFluor Violet 500 SE
NBP2-74199MFV500
mFluor Violet 610 SE
NBP2-74199MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74199
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Sirtuin 5/SIRT5 Antibody (OTI2F1) Summary

Immunogen
Full length human recombinant protein of human SIRT5(NP_036373) produced in HEK293 cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIRT5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
  • Western Blot 1:500-2000
Theoretical MW
33.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.24 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sirtuin 5/SIRT5 Antibody (OTI2F1)

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ36950
  • NAD-dependent deacetylase sirtuin-5
  • silent mating type information regulation 2, S.cerevisiae, homolog 5
  • SIR2L5
  • sir2-like 5
  • SIR2-like protein 5
  • SIRT5
  • sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 5
  • Sirtuin 5
  • sirtuin type 5

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIRT5
Entrez