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S100A8 Antibody (48M7C7) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
48M7C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25269AF350
Alexa Fluor 405
NBP2-25269AF405
Alexa Fluor 488
NBP2-25269AF488
Alexa Fluor 532
NBP2-25269AF532
Alexa Fluor 594
NBP2-25269AF594
Alexa Fluor 647
NBP2-25269AF647
Alexa Fluor 700
NBP2-25269AF700
Alexa Fluor 750
NBP2-25269AF750
Biotin
NBP2-25269B
DyLight 350
NBP2-25269UV
DyLight 405
NBP2-25269V
DyLight 488
NBP2-25269G
DyLight 550
NBP2-25269R
DyLight 594
NBP2-25256DL594
DyLight 650
NBP2-25269C
DyLight 680
NBP2-25269FR
DyLight 755
NBP2-25269IR
FITC
NBP2-25269F
HRP
NBP2-25269H
Janelia Fluor 646
NBP2-25269JF646
Unconjugated
NBP2-25256
mFluor Violet 450 SE
NBP2-25269MFV450
mFluor Violet 500 SE
NBP2-25269MFV500
mFluor Violet 610 SE
NBP2-25269MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-25256
Azide Free
NBP2-25269
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S100A8 Antibody (48M7C7) [Janelia Fluor® 549] Summary

Immunogen
Within the range of amino acids 2-105 of human S100A8 were used as the immunogen for the antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
S100A8
Purity
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for S100A8 Antibody (48M7C7) [Janelia Fluor® 549]

  • 60B8AG
  • CAGACP-10
  • Calgranulin A
  • calgranulin-A
  • Calprotectin L1L subunit
  • CFAG
  • CFAGL1Ag
  • CGLA
  • Cystic fibrosis antigen
  • Leukocyte L1 complex light chain
  • MA387
  • MIF
  • Migration inhibitory factor-related protein 8
  • MRP8
  • MRP-8
  • MRP8S100 calcium binding protein A8 (calgranulin A)
  • NIF
  • P8
  • protein S100-A8
  • S100 calcium binding protein A8
  • S100 calcium-binding protein A8 (calgranulin A)
  • S100 calcium-binding protein A8
  • S100A8
  • Urinary stone protein band A

Background

MRP8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for S100A8/A9 Antibody (NBP2-25269JF549). (Showing 1 - 2 of 2 FAQ).

  1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
    • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
  2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
    • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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Bioinformatics

Gene Symbol S100A8