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RTF1 Antibody

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Independent Antibodies: Western Blot: RTF1 Antibody [NB100-61052] - Detection of Human RTF1 on HeLa whole cell lysate using NB100-61052. RTF1 was also immunoprecipitated by rabbit anti-RTF1 antibody NB600-178.
Immunohistochemistry-Paraffin: RTF1 Antibody [NB100-61052] - Sample: FFPE section of human skin carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RTF1 used at a dilution of 1:1,000 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

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RTF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to the C-terminus of human Restores TBP (TATA box-binding protein) Function-1 using the numbering given in entry EAW92492.1 (GeneID 23168).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RTF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RTF1 Antibody

  • KIAA0252GTL7
  • ortholog of mouse gene trap locus 7
  • RNA polymerase-associated protein RTF1 homolog
  • Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)

Background

In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RTF1 Antibody (NB100-61052) (0)

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Secondary Antibodies

 

Isotype Controls

Additional RTF1 Products

Array NB100-61052

Research Areas for RTF1 Antibody (NB100-61052)

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Bioinformatics

Gene Symbol RTF1
Entrez
Uniprot