Recombinant Human RhoV His Protein Summary
Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-236 of Human RhoV Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMPPRELS EAEPPPLRAP TPPPRRRSAP PELGIKCVLV GDGAVGKSSL IVSYTCNGYP ARYRPTALDT FSVQVLVDGA PVRIELWDTA GQEDFDRLRS LCYPDTDVFL ACFSVVQPSS FQNITEKWLP EIRTHNPQAP VLLVGTQADL RDDVNVLIQL DQGGREGPVP QPQAQGLAEK IRACCYLECS ALTQKNLKEV FDSAILSAIE HKARLEKKLN AKGVRTLSRC RWKKFFCFV |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
RHOV |
Purity |
>85%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
Theoretical MW |
28.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.1 M NaCl, 1 mM DTT |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>85%, by SDS-PAGE |
Alternate Names for Recombinant Human RhoV His Protein
Background
Rho-related GTP-binding protein RhoV, also known as RHOV, is a member of the Rho family and small GTPase superfamily. RHOV is a 236 amino acid protein that controls the actin cytoskeleton through activation of the JNK pathway. RHOV functions as a lipid anchor at the cytoplasmic side of the cell membrane and is expressed in placenta, pancreas and fetal brain. Recombinant human RHOV protein, fused to His-tag at N-terminus, was expressed in E.coli.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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