Recombinant Rat IL-6 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature rat IL-6 is 187 amino acids (aa) in length and shares 39% and 85% aa sequence identity with human and mouse IL-6, respectively IL-6 (6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 9). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 10).

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Developmet, In Vivo.

Showing Publications 1 - 10 of 25. Show All 25 Publications. Collapse Publications.

Publications using 506-RL/CF	Applications	Species
YT Zhao, J Deng, HM Liu, JY Wei, HT Fan, M Liu, T Xu, TF Chen, JY He, WM Sun, TY Jia, XQ Zhang, WJ Xin Adaptation of prelimbic cortex mediated by IL-6/STAT3/Acp5 pathway contributes to the comorbidity of neuropathic pain and depression in rats Journal of Neuroinflammation, 2022-06-11;19(1):144. 2022-06-11 [PMID: 35690777] (Bioassay, Rat)	Bioassay	Rat
X Ding, W Yang, Q Ren, J Hu, S Yang, W Han, J Wang, X Wang, H Wang Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 2020-06-16;17(1):190. 2020-06-16 [PMID: 32546235] (Bioassay, Rat)	Bioassay	Rat
M Alhouayek, L Stafberg, J Karlsson, SH Bergström, CJ Fowler Effects of orthotopic implantation of rat prostate tumour cells upon components of the N-acylethanolamine and monoacylglycerol signalling systems: an mRNA study Sci Rep, 2020-04-14;10(1):6314. 2020-04-14 [PMID: 32286386] (Bioassay, Rat)	Bioassay	Rat
W Chen, H Yuan, W Cao, T Wang, W Chen, H Yu, Y Fu, B Jiang, H Zhou, H Guo, X Zhao Blocking interleukin-6 trans-signaling protects against renal fibrosis by suppressing STAT3 activation Theranostics, 2019-05-31;9(14):3980-3991. 2019-05-31 [PMID: 31281526] (Bioassay, Rat)	Bioassay	Rat
Q Li, Q Cao, C Wang, H Nguyen, XM Wang, G Zheng, YM Wang, S Hu, SI Alexander, DCH Harris, Y Wang Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis J. Leukoc. Biol., 2019-02-27;0(0):. 2019-02-27 [PMID: 30811635] (Bioassay, Mouse)	Bioassay	Mouse
P Dubový, I Klusáková, I Hradilová-, V Brázda, M Kohoutková, M Joukal A Conditioning Sciatic Nerve Lesion Triggers a Pro-regenerative State in Primary Sensory Neurons Also of Dorsal Root Ganglia Non-associated With the Damaged Nerve Front Cell Neurosci, 2019-02-04;13(0):11. 2019-02-04 [PMID: 30778286] (In Vivo, Rat)	In Vivo	Rat
T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat J Pharmacol Toxicol Methods, 2018-01-31;91(0):43-49. 2018-01-31 [PMID: 29371052] (ELISA (Standard), Rat)	ELISA (Standard)	Rat
Levy O, Calippe B, Lavalette S, Hu S, Raoul W, Dominguez E, Housset M, Paques M, Sahel J, Bemelmans A, Combadiere C, Guillonneau X, Sennlaub F Apolipoprotein E promotes subretinal mononuclear phagocyte survival and chronic inflammation in age-related macular degeneration. EMBO Mol Med, 2015-02-01;7(2):211-26. 2015-02-01 [PMID: 25604058] (In Vivo, Mouse)	In Vivo	Mouse
Tsukahara T, Watanabe K, Watanabe T, Yamagami H, Sogawa M, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Tumor necrosis factor alpha decreases glucagon-like peptide-2 expression by up-regulating G-protein-coupled receptor 120 in Crohn disease. Am J Pathol, 2014-11-05;185(1):185-96. 2014-11-05 [PMID: 25447053] (Bioassay, Rat)	Bioassay	Rat
Weinkopff T, Mackenzie C, Eversole R, Lammie P Filarial excretory-secretory products induce human monocytes to produce lymphangiogenic mediators. PLoS Negl Trop Dis, 2014-07-10;8(7):e2893. 2014-07-10 [PMID: 25010672] (In Vivo, Rat)	In Vivo	Rat
Zhang H, Yin Y, Wang G, Liu Z, Liu L, Sun F Interleukin-6 disrupts blood-testis barrier through inhibiting protein degradation or activating phosphorylated ERK in Sertoli cells. Sci Rep, 2014-03-03;4(0):4260. 2014-03-03 [PMID: 24584780] (Bioassay, Rat)	Bioassay	Rat
Gupta , Shashi, Hirota , Masao, Waugh , Sheela M, Murakami , Ikuo, Suzuki , Tomoki, Muraguchi , Masahiro, Shibamori , Masafumi, Ishikawa , Yuichi, Jarvis , Thale C, Carter , Jeffrey, Zhang , Chi, Gawande , Bharat, Vrkljan , Michael, Janjic , Nebojsa, Schneider , Daniel J Chemically modified DNA aptamers bind interleukin-6 with high affinity and inhibit signaling by blocking its interaction with interleukin-6 receptor. J Biol Chem, 2014-01-12;289(12):8706-19. 2014-01-12 [PMID: 24415766] (Bioassay)	Bioassay
Zhang J, Li Y, Shan K, Wang L, Qiu W, Lu Y, Zhao D, Zhu G, He F, Wang Y Sublytic C5b-9 induces IL-6 and TGF-beta1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBPbeta acetylation. FASEB J, 2013-12-16;28(3):1511-25. 2013-12-16 [PMID: 24344329] (Bioassay, Rat)	Bioassay	Rat
Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J Neuronal synapse formation induced by microglia and interleukin 10. PLoS ONE, 2013-11-22;8(11):e81218. 2013-11-22 [PMID: 24278397] (Cell Culture, Rat)	Cell Culture	Rat
Steelman A, Smith R, Welsh C, Li J Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis. J Biol Chem, 2013-07-08;288(33):23776-87. 2013-07-08 [PMID: 23836896] (Bioassay, Rat)	Bioassay	Rat
Oh J, McCloskey MA, Blong CC Astrocyte-derived interleukin-6 promotes specific neuronal differentiation of neural progenitor cells from adult hippocampus. J. Neurosci. Res., 2010-10-01;88(13):2798-809. 2010-10-01 [PMID: 20568291] (Bioassay, Rat)	Bioassay	Rat
Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes. Cell. Signal., 2008-10-12;21(1):151-60. 2008-10-12 [PMID: 18950705] (Bioassay, Mouse, Rat)	Bioassay	Mouse, Rat
Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells, 2008;26(4):1047-55. 2008 [PMID: 18192235] (ELISA Developmet, Rat)	ELISA Developmet	Rat
Whitaker KW, Reyes TM Central blockade of melanocortin receptors attenuates the metabolic and locomotor responses to peripheral interleukin-1beta administration. Neuropharmacology, 2007-11-05;54(3):509-20. 2007-11-05 [PMID: 18082228] (In Vivo, Rat)	In Vivo	Rat
Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur. J. Neurosci., 2007-02-01;25(3):649-58. 2007-02-01 [PMID: 17328769] (Bioassay, Rat)	Bioassay	Rat
Roche M, Diamond M, Finn DP In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids. J. Neuroimmunol., 2006-09-29;181(1):57-67. 2006-09-29 [PMID: 17011047] (ELISA (Standard))	ELISA (Standard)
Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006-06-01;15(3):407-21. 2006-06-01 [PMID: 16846377] (Bioassay, Rat)	Bioassay	Rat
Cao Z, Gao Y, Bryson JB, Hou J, Chaudhry N, Siddiq M, Martinez J, Spencer T, Carmel J, Hart RB, Filbin MT The cytokine interleukin-6 is sufficient but not necessary to mimic the peripheral conditioning lesion effect on axonal growth. J. Neurosci., 2006-05-17;26(20):5565-73. 2006-05-17 [PMID: 16707807] (Bioassay, In Vivo, Rat)	Bioassay, In Vivo	Rat
Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin. J. Neurochem., 2006-02-10;96(6):1667-79. 2006-02-10 [PMID: 16476078] (Bioassay, Rat)	Bioassay	Rat
Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Lee DC, Prieto J, Avila MA Amphiregulin: an early trigger of liver regeneration in mice. Gastroenterology, 2005-02-01;128(2):424-32. 2005-02-01 [PMID: 15685553] (Bioassay, Rat)	Bioassay	Rat
Show All 25 Publications. Collapse Publications.

Array 506-RL/CF

• IL-6 Antibodies
• IL-6 Antibody Pair
• IL-6 ELISA Kits
• IL-6 Lysate
• IL-6 Proteins
• IL-6 Proteins and Enzymes
• IL-6 ELISAs