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Recombinant Rat IL-1 beta/IL-1F2 Protein, CF

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Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.3-1.5 ng/mL in the presence of concanavalin A.
Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL/CF) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.3-1.5 ng/mL in the presence of concanavalin A.
1 μg/lane of Recombinant Rat IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
501-RL-010
Carrier Free
501-RL-050/CF
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Recombinant Rat IL-1 beta/IL-1F2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.300‑1.50 ng/mL.
Source
E. coli-derived rat IL-1 beta/IL-1F2 protein
Val117-Ser268, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il1b
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
501-RL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat IL-1 beta/IL-1F2 Protein, CF

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65% - 77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta .

  1. Allan, S.M. et al., (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S., (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Accession # M98820.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

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Recombinant Rat
IL-1 beta/IL-1F2 Prot ...
501-RL/CF
Recombinant Rat IL-1 beta/IL-1F2 Protein, CF
Species: Rt
Applications: Bioactivity

Publications for IL-1 beta/IL-1F2 (501-RL/CF)(58)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.


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Binding Assay
(1)
Bioassay
(33)
Cell Culture
(3)
ELISA (Standard)
(3)
In Vivo
(16)
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
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(52)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using 501-RL/CF Applications Species
R Lu, H Xu, X Deng, Y Wang, Z He, S Xu, S Liang, X Huang, H You, F Guo, P Cheng, AM Chen Physalin A alleviates intervertebral disc degeneration via anti-inflammatory and anti-fibrotic effects Journal of Orthopaedic Translation, 2023-01-25;39(0):74-87. 2023-01-25 [PMID: 36788965] (Bioassay, Rat) Bioassay Rat
CMD Mota, CJ Madden Neural circuits mediating circulating interleukin-1beta-evoked fever in the absence of prostaglandin E2 production Brain, Behavior, and Immunity, 2022-04-14;103(0):109-121. 2022-04-14 [PMID: 35429606] (In Vivo, Rat) In Vivo Rat
B Ni, W Pei, Y Qu, R Zhang, X Chu, Y Wang, X Huang, H You MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis Oxidative Medicine and Cellular Longevity, 2021-11-03;2021(0):4139048. 2021-11-03 [PMID: 34777685] (Bioassay, Mouse) Bioassay Mouse
J Bao, C Lin, X Zhou, D Ma, L Ge, K Xu, SAA Moqbel, Y He, C Ma, J Ran, L Wu circFAM160A2 Promotes Mitochondrial Stabilization and Apoptosis Reduction in Osteoarthritis Chondrocytes by Targeting miR-505-3p and SIRT3 Oxidative Medicine and Cellular Longevity, 2021-10-04;2021(0):5712280. 2021-10-04 [PMID: 34646424] (Bioassay, Rat) Bioassay Rat
J Yan, Y Zhang, G Sheng, B Ni, Y Xiao, S Wang, T Wang, Y Ma, H Wang, H Wu, C Tu Selonsertib Alleviates the Progression of Rat Osteoarthritis: An in vitro and in vivo Study Frontiers in Pharmacology, 2021-07-12;12(0):687033. 2021-07-12 [PMID: 34322020] (Bioassay, Rat) Bioassay Rat
K Xu, C Lin, D Ma, M Chen, X Zhou, Y He, SAA Moqbel, C Ma, L Wu Spironolactone Ameliorates Senescence and Calcification by Modulating Autophagy in Rat Tendon-Derived Stem Cells via the NF-kappaB/MAPK Pathway Oxidative Medicine and Cellular Longevity, 2021-06-30;2021(0):5519587. 2021-06-30 [PMID: 34306308] (Bioassay, Rat) Bioassay Rat
GY Min, JM Park, IH Joo, DH Kim Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs, NF-&amp;kappaB signaling pathway International Journal of Medical Sciences, 2021-01-01;18(4):861-872. 2021-01-01 [PMID: 33456343] (Bioassay, Rat) Bioassay Rat
SA Rajput, MR Mirza, MI Choudhary Bergenin protects pancreatic beta cells against cytokine-induced apoptosis in INS-1E cells PLoS ONE, 2020-12-21;15(12):e0241349. 2020-12-21 [PMID: 33347462] (Bioassay, Rat) Bioassay Rat
C Lian, T Tao, P Su, Z Liao, X Wang, Y Lei, P Zhao, L Liu Targeting miR-18a sensitizes chondrocytes to anticytokine therapy to prevent osteoarthritis progression Cell Death Dis, 2020-11-03;11(11):947. 2020-11-03 [PMID: 33144571] (In Vivo, Rat) In Vivo Rat
J Ge, X Cheng, Q Yan, C Wu, Y Wang, H Yu, H Yang, F Zhou, J Zou Calcitonin inhibits intervertebral disc degeneration by regulating protein kinase C J. Cell. Mol. Med., 2020-06-21;0(0):. 2020-06-21 [PMID: 32564456] (Bioassay, Rat) Bioassay Rat
Show All 58 Publications.

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By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.
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Bioinformatics

Gene Symbol Il1b
Uniprot