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Recombinant Rat CXCL3/CINC-2 alpha Protein

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
516-CA-025/CF
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Recombinant Rat CXCL3/CINC-2 alpha Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 3-6 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 4-20 ng/mL.
Source
E. coli-derived rat CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Arg33-Ser100
Accession #
N-terminal Sequence
Arg33
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
7.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CXCL3/CINC-2 alpha Protein

  • chemokine (C-X-C motif) ligand 3
  • CINC-2
  • CINC-2b
  • C-X-C motif chemokine 3
  • CXCL3
  • Dcip1
  • Gm1960
  • GRO gamma
  • GRO3 oncogene
  • GRO3
  • GROG
  • GRO-gamma
  • GRO-gamma(1-73)
  • Growth-regulated protein gamma
  • Macrophage inflammatory protein 2-beta
  • melanoma growth stimulatory activity gamma
  • MGSA gamma
  • MIP2B
  • MIP-2b
  • MIP2-beta
  • SCYB3

Background

The rat chemokines CINC (cytokine-induced neutrophil chemoattractant)-1, CINC-2 alpha , CINC-2 beta and CINC-3 (also named MIP-2) constitute a group of rat CXC chemokines that show significant sequence similarity to human GROs and mouse MIP-2 but not IL-8. CINC-2 alpha , CINC-2 beta were originally purified as novel neutrophil chemoattractants from the conditioned medium of rat granulation tissue which also contain CINC-1 and CINC-3. Based on amino acid (aa) sequence analysis of the purified CINC-2 alpha protein and sequencing of the CINC-2 beta cDNA clone, both mature CINC-2 alpha and CINC-2 beta were shown to contain 68 aa residues. The amino acid sequences of the two CINC-2 proteins are identical except for three carboxy-terminal residues. CINC-2 beta cDNA encodes a 100 aa residue precursor protein with a 32 aa residue signal peptide that is removed to yield the mature secreted protein. At the protein sequence level, mature CINC-2 proteins are 63% identical to CINC-1 and 80% identical to CINC-3. CINC-2 proteins represent the major chemokines purified from conditioned medium of granulation tissue or LPS-induced inflammatory exudate. Other cell types known to produce CINC-2 proteins include activated macrophages and fibroblasts.

Recombinant and natural CINC-2 proteins have been shown to be specific neutrophil chemoattractants both in vivo and in vitro. On the basis of cross-desensitization results of the various CINC proteins, it has been postulated that rat neutrophils have at least two classes of CINC receptors: a class of CINC-3-specific receptor as well as a second common receptor shared by all CINCs.

  1. Nakagawa, H. et al. (1994) Biochem. J. 301:545.
  2. Watanabe, K. et al. (1989) J. Biol. Chem. 264:19559.
  3. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
  4. Shibata, F. et al. (1995) Eur. J. Biochem. 231:306.
  5. Al-Mokdad, M. et al. (1996) Biol. Pharm. Bull. 19:879.
  6. Murakami, K. et al. (1997) Biochem. and Biophys. Res. Commun. 232:562.
  7. Watanabe, K. et al. (1991) Exp. Mol. Pathol. 55:30.
  8. Nakagawa, H. et al. (1996) Biochem. and Biophys. Res. Commun. 220:945.

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Bioinformatics

Gene Symbol Cxcl3
Uniprot