Home » Betacellulin/BTC » Betacellulin/BTC Proteins » Recombinant Mouse Betacellulin Protein
Novus Biologicals products are now on bio-techne.com

Recombinant Mouse Betacellulin Protein

 (5 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for Betacellulin/BTC (1025-CE).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
1025-CE-025/CF
Carrier Free
1025-CE-025/CF
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Mouse Betacellulin Protein Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.6 ng/mL.
Source
E. coli-derived mouse Betacellulin/BTC protein
Asp32-Gln118
Accession #
N-terminal Sequence
Asp32
Protein/Peptide Type
Recombinant Proteins
Gene
Btc
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
9.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1025-CE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Betacellulin Protein

  • Betacellulin
  • BTC
  • probetacellulin

Background

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha , amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

  1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
  2. Shing, Y. et al. (1993) Science 259:1604.
  3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
  4. Pathak, B.G. et al. (1995) Genomics 28:116.
  5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
  6. Li, L. et al. (2001) Endocrinology 142:5379.

Customers Who Viewed This Item Also Viewed...

EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(791 Publications)
Species: Hu
Applications: BA
Amphiregulin
Antibody
AF262
Neutralization Amphiregulin Antibody
Immunocytochemistry Amphiregulin Antibody
Immunohistochemistry Amphiregulin Antibody
(1 Review)
(35 Publications)
Species: Hu
Applications: ICC, IHC, Neut, WB
ErbB4/Her4
Antibody (182803) [Un ...
MAB1131
Immunocytochemistry ErbB4/Her4 Antibody (182803) [Unconjugated]
Immunohistochemistry ErbB4/Her4 Antibody (182803) [Unconjugated]
(5 Publications)
Species: Hu
Applications: ICC, IHC, WB
Epiregulin
Antibody [Unconjugate
AF1195
Immunohistochemistry Epiregulin Antibody [Unconjugated]
ELISA Epiregulin Antibody [Unconjugated]
Neutralization Epiregulin Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ELISA, IHC, Neut, WB
HB-EGF Antibody
[Unconjugated]
AF-259-NA
Immunohistochemistry HB-EGF Antibody [Unconjugated]
Neutralization HB-EGF Antibody [Unconjugated]
Immunohistochemistry HB-EGF Antibody [Unconjugated]
(57 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
ErbB2/Her2
[Unconjugated]
1129-ER
SDS-PAGE ErbB2/Her2 [Unconjugated]
Bioactivity ErbB2/Her2 [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
HB-EGF Antibody
[Unconjugated]
AF-259-NA
Immunohistochemistry HB-EGF Antibody [Unconjugated]
Neutralization HB-EGF Antibody [Unconjugated]
Immunohistochemistry HB-EGF Antibody [Unconjugated]
(57 Publications)
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, WB
ErbB3/Her3
Antibody (66223) [Unc ...
MAB3481
Neutralization ErbB3/Her3 Antibody (66223) [Unconjugated]
(21 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, Neut
TGF-alpha
[Unconjugated]
239-A
TGF-alpha [Unconjugated]
(58 Publications)
Species: Hu
Applications: BA
Neuregulin-1/NRG1
Antibody (147724) [Un ...
MAB377
Neuregulin-1/NRG1 Antibody (147724) [Unconjugated]
(84 Publications)
Species: Hu
Applications: WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free
(14 Publications)
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
Activin A
[Unconjugated]
338-AC
Activin A [Unconjugated]
(529 Publications)
Species: Hu, Mu, Rt
Applications: BA
Ferredoxin
Reductase Antibody
NBP2-38530
Western Blot Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Immunohistochemistry-Paraffin Ferredoxin Reductase Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AKR1B1 Antibody
NBP1-89146
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Immunohistochemistry-Paraffin AKR1B1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PDX-1/IPF1
Antibody [Unconjugate
AF2419
Western Blot PDX-1/IPF1 Antibody [Unconjugated]
Immunocytochemistry PDX-1/IPF1 Antibody [Unconjugated]
Simple Western PDX-1/IPF1 Antibody [Unconjugated]
(70 Publications)
Species: Hu
Applications: Dual ISH-IHC, ICC, IHC, Simple Western, WB

Publications for Betacellulin/BTC (1025-CE)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 1025-CE Applications Species
F Westendorp, ON Karpus, PJ Koelink, JLM Vermeulen, S Meisner, J Koster, NVJA Büller, ME Wildenberg, V Muncan, GR van den Br Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation Oncogene, 2021-01-21;0(0):. 2021-01-21 [PMID: 33479497] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 2017-08-07;0(0):. 2017-08-07 [PMID: 28783046] (In Vivo, Mouse) In Vivo Mouse
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 2015-01-02;194(3):1039-46. 2015-01-02 [PMID: 25556244] (Bioassay, Mouse) Bioassay Mouse
Yilmaz M, Maass D, Tiwari N, Waldmeier L, Schmidt P, Lehembre F, Christofori G Transcription factor Dlx2 protects from TGFbeta-induced cell-cycle arrest and apoptosis. EMBO J., 2011-09-06;30(21):4489-99. 2011-09-06 [PMID: 21897365] (Bioassay, Mouse) Bioassay Mouse
Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells. Biochem. Biophys. Res. Commun., 2007-12-04;366(1):129-34. 2007-12-04 [PMID: 18060872] (Bioassay, Human) Bioassay Human

Reviews for Betacellulin/BTC (1025-CE) (0)

There are no reviews for Betacellulin/BTC (1025-CE). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Betacellulin/BTC (1025-CE) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Betacellulin/BTC Products

Blogs on Betacellulin/BTC

There are no specific blogs for Betacellulin/BTC, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse Betacellulin Protein and receive a gift card or discount.


Bioinformatics

Gene Symbol Btc
Uniprot