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Recombinant Mouse Betacellulin Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Betacellulin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 0.15-0.6 ng/mL.
Source
E. coli-derived mouse Betacellulin/BTC protein
Asp32-Gln118
Accession #
N-terminal Sequence
Asp32
Protein/Peptide Type
Recombinant Proteins
Gene
Btc
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
9.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1025-CE/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Betacellulin Protein, CF

  • Betacellulin
  • BTC
  • probetacellulin

Background

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha , amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

  1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
  2. Shing, Y. et al. (1993) Science 259:1604.
  3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
  4. Pathak, B.G. et al. (1995) Genomics 28:116.
  5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
  6. Li, L. et al. (2001) Endocrinology 142:5379.

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Publications for Betacellulin/BTC (1025-CE/CF)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(4)
In Vivo
(1)
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Human
(1)
Mouse
(3)
Transgenic Mouse
(1)
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Showing Publications 1 - 5 of 5.
Publications using 1025-CE/CF Applications Species
F Westendorp, ON Karpus, PJ Koelink, JLM Vermeulen, S Meisner, J Koster, NVJA Büller, ME Wildenberg, V Muncan, GR van den Br Epithelium-derived Indian Hedgehog restricts stromal expression of ErbB family members that drive colonic tumor cell proliferation Oncogene, 2021-01-21;0(0):. 2021-01-21 [PMID: 33479497] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
LJ Martin, SB Smith, A Khoutorsky, CA Magnussen, A Samoshkin, RE Sorge, C Cho, N Yosefpour, S Sivaselvac, S Tohyama, T Cole, TM Khuong, E Mir, DG Gibson, JS Wieskopf, SG Sotocinal, JS Austin, CB Meloto, JH Gitt, C Gkogkas, N Sonenberg, JD Greenspan, RB Fillingim, R Ohrbach, GD Slade, C Knott, R Dubner, AG Nackley, A Ribeiro-da, GG Neely, W Maixner, DV Zaykin, JS Mogil, L Diatchenko Epiregulin and EGFR interactions are involved in pain processing J. Clin. Invest., 2017-08-07;0(0):. 2017-08-07 [PMID: 28783046] (In Vivo, Mouse) In Vivo Mouse
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 2015-01-02;194(3):1039-46. 2015-01-02 [PMID: 25556244] (Bioassay, Mouse) Bioassay Mouse
Yilmaz M, Maass D, Tiwari N, Waldmeier L, Schmidt P, Lehembre F, Christofori G Transcription factor Dlx2 protects from TGFbeta-induced cell-cycle arrest and apoptosis. EMBO J., 2011-09-06;30(21):4489-99. 2011-09-06 [PMID: 21897365] (Bioassay, Mouse) Bioassay Mouse
Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells. Biochem. Biophys. Res. Commun., 2007-12-04;366(1):129-34. 2007-12-04 [PMID: 18060872] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol Btc
Uniprot