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Recombinant Human TFF1 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TFF1 Protein, CF Summary

Details of Functionality
Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF1 can effectively induce ERK1/2 phosphorylation.
Source
Chinese Hamster Ovary cell line, CHO-derived human TFF1 protein
Glu25-Phe84 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu25
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TFF1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12-15 kDa, reducing conditions
Publications
Read Publication using
8289-TF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TFF1 Protein, CF

  • BCEI
  • BCEIbreast cancer, estrogen-inducible sequence expressed in
  • Breast cancer estrogen-inducible protein
  • breast cancer estrogen-inducible sequence
  • D21S21
  • gastrointestinal trefoil protein pS2
  • hP1.A
  • HPS2
  • PNR-2
  • Polypeptide P1.A
  • Protein pS2
  • PS2
  • TFF1
  • trefoil factor 1
  • trefoil factor, BCE1, human pS2 induced by estrogen from human breast cancercell line M10HP1.A

Background

Trefoil Factor 1 (TFF1), also known as pS2, is one of three structurally related secreted proteins that contain trefoil domains. These domains adopt a three-leaved conformation held together by conserved intrachain disulfide bonds. TFF1 is an approximately 7 kDa peptide that plays an important role in epithelial regeneration and wound healing (1). Mature human TFF1 shares 67% amino acid sequence identity with mouse and rat TFF1. It is expressed by goblet cells of the gastric and intestinal mucosa and by conjunctival goblet cells (2-5). TFF1 is a copper-binding protein that can form disulfide-linked homodimers, associate into disulfide-linked complexes with Gastrokine 2, and form non-covalent complexes with the mucin MUC5AC (4, 6-8). Copper enhances TFF1 homodimerization as well as its ability to promote epithelial cell motility, wound healing, and the colonization of H. pylori in stomach and colon epithelia (9, 10). TFF1 is down-regulated during the progression from gastritis to gastric dysplasia to gastric cancer, although it is up-regulated in breast and prostate cancers (11-13). TFF1 inhibits the formation of calcium oxalate crystals, and its excretion in the urine is reduced in patients with kidney stones (14).
  1. Kjellev, S. (2009) Cell. Mol. Life Sci. 66:1350.
  2. Madsen, J. et al. (2007) J. Histochem. Cytochem. 55:505.
  3. Rio, M.C. et al. (1988) Science 241:705.
  4. Newton, J.L. et al. (2000) Gut 46:312.
  5. Langer, G. et al. (1999) Invest. Opthalmol. Visual Sci. 40:2220.
  6. Chadwick, M.P. et al. (1997) Biochem. Biophys. Res. Commun. 327:117.
  7. Westley, B.R. et al. (2005) Biochemistry 44:7967.
  8. Ruchaud-Sparagano, M.H. et al. (2004) Cell. Mol. Life Sci. 61:1946.
  9. Tosco, A. et al. (2010) Cell. Mol. Life Sci. 67:1943.
  10. Montefusco, S. et al. (2013) PLoS ONE 8:e79455.
  11. Soutto, M. et al. (2011) J. Clin. Invest. 121:1753.
  12. Kida, N. et al. (1989) Cancer Res. 49:3494.
  13. Vestergaard, E.M. et al. (2006) Clin. Cancer Res. 12:807.
  14. Chutipongtanate, S. et al. (2005) J. Clin. Invest. 115:3613.

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Recombinant Human
TFF1 Protein, CF
8289-TF
Recombinant Human TFF1 Protein, CF
Species: Hu
Applications: Bioactivity

Publications for TFF1/pS2 (8289-TF)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: In vivo assay.


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Bioinformatics

Gene Symbol TFF1
Uniprot