Recombinant Human EphB2 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125 kDa member of the transmembrane Eph receptor tyrosine kinase family that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin (1, 2). Human EphB2 cDNA encodes a 1055 amino acid (aa) precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD), a 21 aa transmembrane segment, and a 491 aa cytoplamic domain. The ECD contains a cysteine-rich region followed by two fibronectin type III domains. The cytoplasmic domain contains the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (3). Human EphB2 shares 99% aa sequence identity with mouse and rat EphB2 within the ECD region. A short isoform that lacks 70 aa at the C-terminus has also been reported (4). Hippocampal neurons can release vesicles containing full length EphB2, and these are taken up by neighboring glial cells (5). EphB2 is expressed on both sides of the neuronal synapse. It controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity
(6-10). EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development (6, 11-15). Signaling in Ephrin expressing cells through EphB2-Ephrin complex requires proteolytic cleavage of EphB2 that releases its extracellular domain (16). Following the shedding of the extracellular domain of EphB2, the cytoplasmic domain of EphB2 is released from the plasma membrane by the presenilin-dependent gamma -secretase activity to initiate a signaling cascade in the EphB2 expressing cells (16). Aberrant EphB2 expression and activity are implicated in the progression of several cancers (17).

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 4 applications: Bioassay, CAR-T (Bioassay), Cell Culture, Enzyme Assay.

Showing Publications 1 - 4 of 4.

Publications using 5189-B2	Applications	Species
Narayanan, KK;Amaya, M;Tsang, N;Yin, R;Jays, A;Broder, CC;Shukla, D;Procko, E; The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins bioRxiv : the preprint server for biology 2023-04-26 [PMID: 37162958] (Bioassay, N/A)	Bioassay	N/A
D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands J. Leukoc. Biol., 2020-04-26;0(0):. 2020-04-26 [PMID: 32337793] (Cell Culture, Human)	Cell Culture	Human
Bielamowicz K, Fousek K, Byrd T, Samaha H, Mukherjee M, Aware N, Wu M, Orange J, Sumazin P, Man T, Joseph S, Hegde M, Ahmed N Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma. Neuro Oncol, 2018-03-27;20(4):506-518. 2018-03-27 [PMID: 29016929] (CAR-T (Bioassay), Human)	CAR-T (Bioassay)	Human
Zhang , Gu, Brady , John, Liang , Wei-Chin, Wu , Yan, Henkemeyer , Mark, Yan , Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015-04-13;6(0):6625. 2015-04-13 [PMID: 25865237] (Enzyme Assay, Human)	Enzyme Assay	Human

Array 5189-B2

• EphB2 Antibodies
• EphB2 Protein
• EphB2 Proteins and Enzymes