Recombinant Human CCL3/MIP-1 alpha Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP‑1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP‑1 beta . Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature human CCL3 shares 70%‑74% amino acid sequence identity with mouse, rat, and cotton rat CCL3 (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently‑linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8‑10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11‑14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1‑mediated noxious stimulation (16).

We have publications tested in 5 confirmed species: Human, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), Hemofiltration Procedure.

Showing Publications 1 - 10 of 38. Show All 38 Publications. Collapse Publications.

Publications using 270-LD/CF	Applications	Species
Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience, 2021-11-14;24(12):103433. 2021-11-14 [PMID: 34917892] (Bioassay, Human)	Bioassay	Human
B Karakaya, CHM van Moorse, M Veltkamp, C Roodenburg, KM Kazemier, AHM van der He, TWJ Huizinga, JC Grutters, GT Rijkers A Polymorphism in C-C Chemokine Receptor 5 (CCR5) Associates with L�fgren's Syndrome and Alters Receptor Expression as well as Functional Response Cells, 2021-08-03;10(8):. 2021-08-03 [PMID: 34440736] (Bioassay, Human)	Bioassay	Human
Y Ishida, Y Kuninaka, M Nosaka, A Kimura, A Taruya, M Furuta, N Mukaida, T Kondo Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis Nat Commun, 2020-11-25;11(1):5994. 2020-11-25 [PMID: 33239616] (Cell Culture, Human)	Cell Culture	Human
T Kodama, YI Koma, N Arai, A Kido, N Urakawa, M Nishio, M Shigeoka, H Yokozaki CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways Lab. Invest., 2020-05-26;0(0):. 2020-05-26 [PMID: 32457351] (Bioassay, Human)	Bioassay	Human
H Nakano, Y Kirino, M Takeno, K Higashitan, H Nagai, R Yoshimi, Y Yamaguchi, I Kato, I Aoki, H Nakajima GWAS-identified CCR1 and IL10 loci contribute to M1 macrophage-predominant inflammation in Beh�et's disease Arthritis Res. Ther., 2018-06-12;20(1):124. 2018-06-12 [PMID: 29895319] (Bioassay, Human)	Bioassay	Human
JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism Blood, 2018-03-01;0(0):. 2018-03-01 [PMID: 29496671] (Bioassay, Human)	Bioassay	Human
MH van Atteku, S Terpstra, E Slinger, M von Linder, PD Moerland, A Jongejan, AP Kater, E Eldering Macrophages confer survival signals via CCR1-dependent translational MCL-1 induction in chronic lymphocytic leukemia Oncogene, 2017-02-13;0(0):. 2017-02-13 [PMID: 28192408] (Bioassay, Human)	Bioassay	Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016-12-02;0(0):. 2016-12-02 [PMID: 27911005] (Hemofiltration Procedure, Human)	Hemofiltration Procedure	Human
Stefanie Karlshøj Molecular Mechanism of Action for Allosteric Modulators and Agonists in CC-chemokine Receptor 5 (CCR5) J. Biol. Chem, 2016-11-10;291(52):26860-26874. 2016-11-10 [PMID: 27834679] (Bioassay)	Bioassay
Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies Mucosal Immunol, 2016-02-17;9(6):1584-1595. 2016-02-17 [PMID: 26883728] (Bioassay, Human)	Bioassay	Human
Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines. Eur J Immunol, 2014-12-01;45(1):101-12. 2014-12-01 [PMID: 25345597] (Bioassay, Human)	Bioassay	Human
Ravi A, Khurana S, Lemon J, Plumb J, Booth G, Healy L, Catley M, Vestbo J, Singh D Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum. Respir Res, 2014-09-04;15(0):103. 2014-09-04 [PMID: 25183374] (Bioassay, Human)	Bioassay	Human
Buttari B, Profumo E, Domenici G, Tagliani A, Ippoliti F, Bonini S, Businaro R, Elenkov I, Rigano R Neuropeptide Y induces potent migration of human immature dendritic cells and promotes a Th2 polarization. FASEB J, 2014-04-03;28(7):3038-49. 2014-04-03 [PMID: 24699455] (Bioassay, Human)	Bioassay	Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Badr G, Lefevre EA, Mohany M Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis. PLoS ONE, 2011-09-01;6(9):e23741. 2011-09-01 [PMID: 21912642] (Bioassay, Human)	Bioassay	Human
Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection. Eur. J. Immunol., 2011-03-07;41(4):963-73. 2011-03-07 [PMID: 21381021] (Bioassay, Human)	Bioassay	Human
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (ELISA (Standard))	ELISA (Standard)
Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B. Immunology, 2009-12-02;129(4):536-46. 2009-12-02 [PMID: 20002784] (Bioassay, Human)	Bioassay	Human
Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes. J. Leukoc. Biol., 2008-06-30;84(4):1109-19. 2008-06-30 [PMID: 18591415] (Bioassay, Human)	Bioassay	Human
Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood, 2008-05-14;112(3):493-503. 2008-05-14 [PMID: 18480427] (Bioassay, Human)	Bioassay	Human
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (ELISA (Standard))	ELISA (Standard)
Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes. Blood, 2008-01-18;111(7):3579-90. 2008-01-18 [PMID: 18203954] (Bioassay, Human)	Bioassay	Human
Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007-11-01;53(11):2010-2. 2007-11-01 [PMID: 18030696] (ELISA (Standard))	ELISA (Standard)
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (ELISA (Standard))	ELISA (Standard)
Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Int. J. Cancer, 2008-02-15;122(4):769-76. 2008-02-15 [PMID: 17935139] (Bioassay, Human)	Bioassay	Human
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (ELISA (Standard), N/A)	ELISA (Standard)	N/A
Jang SW, Kim YS, Kim YR, Sung HJ, Ko J Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1. J. Biol. Chem., 2007-02-12;282(15):11092-100. 2007-02-12 [PMID: 17296613] (Bioassay, Human)	Bioassay	Human
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (ELISA (Standard))	ELISA (Standard)
Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T Interaction between the CCR5 chemokine receptors and microbial HSP70. Eur. J. Immunol., 2006-09-01;36(9):2304-14. 2006-09-01 [PMID: 16909434] (Bioassay, Human)	Bioassay	Human
Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma. Blood, 2006-07-18;108(10):3465-71. 2006-07-18 [PMID: 16849642] (ELISA (Standard), Human)	ELISA (Standard)	Human
Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)	Binding Assay, Bioassay	Virus
Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression. J. Virol., 2006-01-01;80(1):236-45. 2006-01-01 [PMID: 16352548] (ELISA (Standard))	ELISA (Standard)
Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human)	Bioassay	Human
Tay SS, McCormack A, Rose ML Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Transplantation, 2004-10-15;78(7):987-94. 2004-10-15 [PMID: 15480163] (ELISA (Standard))	ELISA (Standard)
Chang WL, Baumgarth N, Yu D, Barry PA Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality. J. Virol., 2004-08-01;78(16):8720-31. 2004-08-01 [PMID: 15280480] (Bioassay, Human)	Bioassay	Human
Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 2002-11-01;169(9):5244-51. 2002-11-01 [PMID: 12391243] (ELISA (Standard))	ELISA (Standard)
Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain. J. Neurochem., 2002-04-01;81(2):257-69. 2002-04-01 [PMID: 12064472] (Binding Assay, Rat)	Binding Assay	Rat
Lehner T, Wang Y, Doyle C, Tao L, Bergmeier LA, Mitchell E, Bogers WM, Heeney J, Kelly CG Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques. Eur. J. Immunol., 1999-08-01;29(8):2427-35. 1999-08-01 [PMID: 10458756] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))	Bioassay	Primate - Macaca mulatta (Rhesus Macaque)
Show All 38 Publications. Collapse Publications.

Array 270-LD/CF

• CCL3/MIP-1 alpha Antibodies
• CCL3/MIP-1 alpha ELISA Kits
• CCL3/MIP-1 alpha Lysate
• CCL3/MIP-1 alpha Proteins
• CCL3/MIP-1 alpha ELISAs
• CCL3/MIP-1 alpha Proteins and Enzymes