![Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30157] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2) [NBP1-30157].](http://images.novusbio.com/fullsize/Raw-264.7-Whole-Cell-Lysate-Western-Blot-NBP1-30157-img0003.jpg "Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30157] - Samples: 25ug whole cell lysate of RAW 264.7 cells treated with T0901317 (lane 1) and untreated (lane 2) [NBP1-30157].")
| Reactivity | MuSpecies Glossary |
| Applications | WB |
| Concentration | 1.0 mg/ml |
| Dilutions |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95C for 5 minutes and rapidly cool. NOTE: Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Large protein samples should be heated to 65C for 5-10 minutes prior to loading. |
| Control |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added. |
| Concentration | 1.0 mg/ml |
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